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DOI | 10.1111/gcb.13371 |
3D structures of individual mammalian genomes studied by single-cell Hi-C | |
Stevens, Tim J.1; 39;Shaughnessy-Kirwan, Aoife2 | |
2017-04-06 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 544期号:7648页码:59-+ |
文章类型 | Article |
语种 | 英语 |
国家 | England; Spain; Austria; USA; Switzerland |
英文摘要 | The folding of genomic DNA from the beads-on-a-string-like structure of nucleosomes into higher-order assemblies is crucially linked to nuclear processes. Here we calculate 3D structures of entire mammalian genomes using data from a new chromosome conformation capture procedure that allows us to first image and then process single cells. The technique enables genome folding to be examined at a scale of less than 100 kb, and chromosome structures to be validated. The structures of individual topological-associated domains and loops vary substantially from cell to cell. By contrast, A and B compartments, lamina-associated domains and active enhancers and promoters are organized in a consistent way on a genome-wide basis in every cell, suggesting that they could drive chromosome and genome folding. By studying genes regulated by pluripotency factor and nucleosome remodelling deacetylase (NuRD), we illustrate how the determination of single-cell genome structure provides a new approach for investigating biological processes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000398323300032 |
WOS关键词 | CHROMOSOME CONFORMATION CAPTURE ; NUCLEAR LAMINA INTERACTIONS ; EMBRYONIC STEM-CELLS ; SPATIAL-ORGANIZATION ; PLURIPOTENCY FACTORS ; LINEAGE COMMITMENT ; CHROMATIN DOMAINS ; GENE-EXPRESSION ; TRANSCRIPTION ; REVEALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/17127 |
专题 | 气候变化 资源环境科学 |
作者单位 | 1.Univ Cambridge, Dept Biochem, 80 Tennis Court Rd, Cambridge CB2 1GA, England; 2.MRC, Mol Biol Lab, Francis Crick Ave,Cambridge Biomed Campus, Cambridge CB2 0QH, England; 3.Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England; 4.Univ Cambridge, Wellcome Trust MRC Stem Cell Inst, Tennis Court Rd, Cambridge CB2 1QR, England; 5.Ctr Genom Regulat CRG, EMBL CRG Syst Biol Unit, Barcelona 08003, Spain; 6.Univ Pompeu Fabra, Barcelona 08003, Spain; 7.ICREA, Barcelona 08010, Spain; 8.Univ Vienna, Max F Perutz Labs, Vienna Bioctr, Dr Bohr Gasse 9-3, A-1030 Vienna, Austria; 9.Univ Miami, Miller Sch Med, Dept Human Genet, Sylvester Comprehens Canc Ctr, Miami, FL 33136 USA; 10.Swiss Fed Inst Technol, Inst Mol Hlth Sci, HPL E 12,Otto Stern Weg 7, Zurich, Switzerland |
推荐引用方式 GB/T 7714 | Stevens, Tim J.,39;Shaughnessy-Kirwan, Aoife. 3D structures of individual mammalian genomes studied by single-cell Hi-C[J]. NATURE,2017,544(7648):59-+. |
APA | Stevens, Tim J.,&39;Shaughnessy-Kirwan, Aoife.(2017).3D structures of individual mammalian genomes studied by single-cell Hi-C.NATURE,544(7648),59-+. |
MLA | Stevens, Tim J.,et al."3D structures of individual mammalian genomes studied by single-cell Hi-C".NATURE 544.7648(2017):59-+. |
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