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DOI | 10.1126/science.aal2130 |
Assembly of a nucleus-like structure during viral replication in bacteria | |
Chaikeeratisak, Vorrapon1; Katrina Nguyen1; Khanna, Kanika1; Brilot, Axel F.2,3; Erb, Marcella L.1; Coker, Joanna K. C.1; Vavilina, Anastasia1; Newton, Gerald L.1; Buschauer, Robert4; Pogliano, Kit1; Villa, Elizabeth4; Agard, David A.2,3; Pogliano, Joe1 | |
2017-01-13 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6321页码:194-197 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlororaphis phage 201 phi 2-1 assembled a compartment that separated viral DNA from the cytoplasm. The phage compartment was centered by a bipolar tubulin-based spindle, and it segregated phage and bacterial proteins according to function. Proteins involved in DNA replication and transcription localized inside the compartment, whereas proteins involved in translation and nucleotide synthesis localized outside. Later during infection, viral capsids assembled on the cytoplasmic membrane and moved to the surface of the compartment for DNA packaging. Ultimately, viral particles were released from the compartment and the cell lysed. These results demonstrate that phages have evolved a specialized structure to compartmentalize viral replication. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391743700046 |
WOS关键词 | CELL ; MECHANISM ; DNA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195209 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA; 2.Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA; 3.Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA; 4.Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Chaikeeratisak, Vorrapon,Katrina Nguyen,Khanna, Kanika,et al. Assembly of a nucleus-like structure during viral replication in bacteria[J]. SCIENCE,2017,355(6321):194-197. |
APA | Chaikeeratisak, Vorrapon.,Katrina Nguyen.,Khanna, Kanika.,Brilot, Axel F..,Erb, Marcella L..,...&Pogliano, Joe.(2017).Assembly of a nucleus-like structure during viral replication in bacteria.SCIENCE,355(6321),194-197. |
MLA | Chaikeeratisak, Vorrapon,et al."Assembly of a nucleus-like structure during viral replication in bacteria".SCIENCE 355.6321(2017):194-197. |
条目包含的文件 | 条目无相关文件。 |
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