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DOI | 10.1126/science.aag3180 |
Pyocyanin degradation by a tautomerizing demethylase inhibits Pseudomonas aeruginosa biofilms | |
Costa, Kyle C.1; Glasser, Nathaniel R.1; Conway, Stuart J.2; Newman, Dianne K.1,3 | |
2017-01-13 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6321页码:170-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | The opportunistic pathogen Pseudomonas aeruginosa produces colorful redox-active metabolites called phenazines, which underpin biofilm development, virulence, and clinical outcomes. Although phenazines exist in many forms, the best studied is pyocyanin. Here, we describe pyocyanin demethylase (PodA), a hitherto uncharacterized protein that oxidizes the pyocyanin methyl group to formaldehyde and reduces the pyrazine ring via an unusual tautomerizing demethylation reaction. Treatment with PodA disrupts P. aeruginosa biofilm formation similarly to DNase, suggesting interference with the pyocyanin-dependent release of extracellular DNA into the matrix. PodA-dependent pyocyanin demethylation also restricts established biofilm aggregate populations experiencing anoxic conditions. Together, these results show that modulating extracellular redox-active metabolites can influence the fitness of a biofilm-forming microorganism. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391743700040 |
WOS关键词 | STRUCTURE PREDICTION ; EXTRACELLULAR DNA ; REDOX ; BIOCHEMISTRY ; ANTIBIOTICS ; PATHWAYS ; BACTERIA ; OXYGEN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195223 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA; 2.Univ Oxford, Dept Chem, Chem Res Lab, Mansfield Rd, Oxford OX1 3TA, England; 3.CALTECH, Div Geol & Planetary Sci, Pasadena, CA 91125 USA |
推荐引用方式 GB/T 7714 | Costa, Kyle C.,Glasser, Nathaniel R.,Conway, Stuart J.,et al. Pyocyanin degradation by a tautomerizing demethylase inhibits Pseudomonas aeruginosa biofilms[J]. SCIENCE,2017,355(6321):170-+. |
APA | Costa, Kyle C.,Glasser, Nathaniel R.,Conway, Stuart J.,&Newman, Dianne K..(2017).Pyocyanin degradation by a tautomerizing demethylase inhibits Pseudomonas aeruginosa biofilms.SCIENCE,355(6321),170-+. |
MLA | Costa, Kyle C.,et al."Pyocyanin degradation by a tautomerizing demethylase inhibits Pseudomonas aeruginosa biofilms".SCIENCE 355.6321(2017):170-+. |
条目包含的文件 | 条目无相关文件。 |
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