Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells

doi:10.1126/science.aag1927

GSTDTAP > 地球科学

DOI	10.1126/science.aag1927
	Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells
	Choi, Yong Jin 1; Lin, Chao-Po 1; Risso, Davide 2; Chen, Sean 1; Kim, Thomas Aquinas 1; Tan, Meng How 3; Li, Jin Billy 3; Wu, Yalei 4; Chen, Caifu 5; Xuan, Zhenyu 6; Macfarlan, Todd 7; Peng, Weiqun 8; Lloyd, K. C. Kent 9; Kim, Sang Yong 10; Speed, Terence P.11,12,13; He, Lin 1
	2017-02-10
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2017
卷号	355 期号:6325
文章类型	Article
语种	英语
国家	USA; Australia
英文摘要	Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) efficiently generate all embryonic cell lineages but rarely generate extraembryonic cell types. We found that microRNA miR-34a deficiency expands the developmental potential of mouse pluripotent stem cells, yielding both embryonic and extraembryonic lineages and strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features of totipotent two-cell blastomeres. miR-34a restricts the acquisition of expanded cell fate potential in pluripotent stem cells, and it represses MERVL expression through transcriptional regulation, at least in part by targeting the transcription factor Gata2. Our studies reveal a complex molecular network that defines and restricts pluripotent developmental potential in cultured ESCs and iPSCs.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000393636700040
WOS关键词	STAGE MOUSE BLASTOMERES ; GENE-EXPRESSION ; MUERV-L ; EMBRYOS ; DIFFERENTIATION ; P53 ; CONTRIBUTES ; APOPTOSIS ; ENDODERM ; BIOLOGY
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/195406
专题	地球科学资源环境科学气候变化
作者单位	1.Univ Calif Berkeley, Div Cellular & Dev Biol, Dept Mol & Cell Biol, Berkeley, CA 94705 USA; 2.Univ Calif Berkeley, Sch Publ Hlth, Div Biostat, Berkeley, CA 94720 USA; 3.Stanford Univ, Dept Genet, Stanford, CA 94305 USA; 4.Thermo Fisher Sci, 180 Oyster Point Blvd, San Francisco, CA 94080 USA; 5.Integrated DNA Technol, 200 Chesapeake Dr, Redwood City, CA 94063 USA; 6.Univ Texas Dallas, Dept Mol & Cell Biol, Richardson, TX 75080 USA; 7.Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Bethesda, MD 20892 USA; 8.George Washington Univ, Dept Phys, Washington, DC 20052 USA; 9.Univ Calif Davis, Mouse Biol Program, Davis, CA 95616 USA; 10.NYU, Sch Med, Dept Pathol, New York, NY 10016 USA; 11.Univ Calif Berkeley, Dept Stat, Berkeley, CA 94720 USA; 12.Univ Melbourne, Dept Math & Stat, Parkville, Vic 3010, Australia; 13.Walter & Eliza Hall Inst Med Res, Bioinformat Div, Parkville, Vic 3052, Australia
推荐引用方式 GB/T 7714	Choi, Yong Jin,Lin, Chao-Po,Risso, Davide,et al. Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells[J]. SCIENCE,2017,355(6325).
APA	Choi, Yong Jin.,Lin, Chao-Po.,Risso, Davide.,Chen, Sean.,Kim, Thomas Aquinas.,...&He, Lin.(2017).Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells.SCIENCE,355(6325).
MLA	Choi, Yong Jin,et al."Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells".SCIENCE 355.6325(2017).