Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq

doi:10.1126/science.aai8478

GSTDTAP > 地球科学

DOI	10.1126/science.aai8478
	Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq
	Venteicher, Andrew S.1,2,3,4,5; Tirosh, Itay 4; Hebert, Christine 1,2,3,4; Yizhak, Keren 1,2,3,4; Neftel, Cyril 1,2,3,4,6; Filbin, Mariella G.1,2,3,4,7,8; Hovestadt, Volker 1,2,3,4; Escalante, Leah E.1,2,3,4; Shaw, McKenzie L.1,2,3,4; Rodman, Christopher 4; Gillespie, Shawn M.1,2,3; Dionne, Danielle 4; Luo, Christina C.1,2,3; Ravichandran, Hiranmayi 1,2,3; Mylvaganam, Ravindra 1,2,3; Mount, Christopher 9,10,11,12; Onozato, Maristela L.1,2,3; Nahed, Brian V.3,5; Wakimoto, Hiroaki 3,5; Curry, William T.3,5; Iafrate, A. John 1,2,3; Rivera, Miguel N.1,2,3,4; Frosch, Matthew P.1,2,3; Golub, Todd R.4,7,8,13; Brastianos, Priscilla K.3,14,15; Getz, Gad 1,2,3,4; Patel, Anoop P.3,5; Monje, Michelle 9,10,11,12; Cahill, Daniel P.3,5; Rozenblatt-Rosen, Orit 4; Louis, David N.1,2,3; Bernstein, Bradley E.1,2,3,4; Regev, Aviv 4,13,16,17; Suva, Mario L.1,2,3,4
	2017-03-31
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2017
卷号	355 期号:6332
文章类型	Article
语种	英语
国家	USA; Switzerland
英文摘要	Tumor subclasses differ according to the genotypes and phenotypes of malignant cells as well as the composition of the tumor microenvironment (TME). We dissected these influences in isocitrate dehydrogenase (IDH)-mutant gliomas by combining 14,226 single-cell RNA sequencing (RNA-seq) profiles from 16 patient samples with bulk RNA-seq profiles from 165 patient samples. Differences in bulk profiles between IDH-mutant astrocytoma and oligodendroglioma can be primarily explained by distinct TME and signature genetic events, whereas both tumor types share similar developmental hierarchies and lineages of glial differentiation. As tumor grade increases, we find enhanced proliferation of malignant cells, larger pools of undifferentiated glioma cells, and an increase in macrophage over microglia expression programs in TME. Our work provides a unifying model for IDH-mutant gliomas and a general framework for dissecting the differences among human tumor subclasses.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000397809500034
WOS关键词	REVEALS ; HETEROGENEITY ; LANDSCAPES ; GENOMES ; TISSUE ; GENES ; P53
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/195730
专题	地球科学资源环境科学气候变化
作者单位	1.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA; 2.Massachusetts Gen Hosp, Ctr Canc Res, Boston, MA 02114 USA; 3.Harvard Med Sch, Boston, MA 02114 USA; 4.Broad Inst Harvard & MIT, Cambridge, MA 02142 USA; 5.Massachusetts Gen Hosp, Dept Neurosurg, Boston, MA 02114 USA; 6.CHU Vaudois, Fac Biol & Med, Inst Pathol, CH-1011 Lausanne, Switzerland; 7.Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02215 USA; 8.Childrens Hosp, Ctr Canc, Boston, MA 02215 USA; 9.Stanford Univ, Dept Neurol, Sch Med, Stanford, CA 94305 USA; 10.Stanford Univ, Dept Neurosurg, Sch Med, Stanford, CA 94305 USA; 11.Stanford Univ, Dept Pediat, Sch Med, Stanford, CA 94305 USA; 12.Stanford Univ, Dept Pathol, Sch Med, Stanford, CA 94305 USA; 13.Howard Hughes Med Inst, Chevy Chase, MD 20815 USA; 14.Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA; 15.Massachusetts Gen Hosp, Dept Neurol, Boston, MA 02114 USA; 16.MIT, Koch Inst, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 17.MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA
推荐引用方式 GB/T 7714	Venteicher, Andrew S.,Tirosh, Itay,Hebert, Christine,et al. Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq[J]. SCIENCE,2017,355(6332).
APA	Venteicher, Andrew S..,Tirosh, Itay.,Hebert, Christine.,Yizhak, Keren.,Neftel, Cyril.,...&Suva, Mario L..(2017).Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.SCIENCE,355(6332).
MLA	Venteicher, Andrew S.,et al."Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq".SCIENCE 355.6332(2017).