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DOI | 10.1126/science.aai9372 |
Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G(1)/S phase transition | |
Elbarbary, Reyad A.1,2; Miyoshi, Keita1,2; Myers, Jason R.3; Du, Peicheng4; Ashton, John M.3; Tian, Bin5; Maquat, Lynne E.1,2,6 | |
2017-05-26 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6340页码:859-862 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression. The pathways that mediate mature miRNA decay are less well understood than those that mediate miRNA biogenesis. We found that functional miRNAs are degraded in human cells by the endonuclease Tudor-SN (TSN). In vitro, recombinant TSN initiated the decay of both protein-free and Argonaute 2-loaded miRNAs via endonucleolytic cleavage at CA and UA dinucleotides, preferentially at scissile bonds located more than five nucleotides away from miRNA ends. Cellular targets of TSN-mediated decay defined using microRNA sequencing followed this rule. Inhibiting TSN-mediated miRNA decay by CRISPR-Cas9 knockout of TSN inhibited cell cycle progression by up-regulating a cohort of miRNAs that down-regulates mRNAs that encode proteins critical for the G(1)-to-S phase transition. Our study indicates that targeting TSN nuclease activity could inhibit pathological cell proliferation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000401957900041 |
WOS关键词 | STAPHYLOCOCCAL NUCLEASE ; FEEDBACK LOOP ; RNA ; EXPRESSION ; CANCER ; CYCLE ; REGULATOR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196133 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA; 2.Univ Rochester, Ctr RNA Biol, Rochester, NY 14642 USA; 3.Univ Rochester, Genom Res Ctr, Rochester, NY 14642 USA; 4.Rutgers State Univ, Off Adv Res Comp, Piscataway, NJ 08854 USA; 5.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA; 6.Univ Rochester, Sch Med & Dent, Dept Oncol, Rochester, NY 14642 USA |
推荐引用方式 GB/T 7714 | Elbarbary, Reyad A.,Miyoshi, Keita,Myers, Jason R.,et al. Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G(1)/S phase transition[J]. SCIENCE,2017,356(6340):859-862. |
APA | Elbarbary, Reyad A..,Miyoshi, Keita.,Myers, Jason R..,Du, Peicheng.,Ashton, John M..,...&Maquat, Lynne E..(2017).Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G(1)/S phase transition.SCIENCE,356(6340),859-862. |
MLA | Elbarbary, Reyad A.,et al."Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G(1)/S phase transition".SCIENCE 356.6340(2017):859-862. |
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