Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aan0063 |
Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading (vol 357, pg 314, 2017) | |
Coster, Gideon; Diffley, John F. X. | |
2017-07-21 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6348页码:314-318 |
文章类型 | Article |
语种 | 英语 |
国家 | England |
英文摘要 | Bidirectional replication from eukaryotic DNA replication origins requires the loading of two ring-shaped minichromosome maintenance (MCM) helicases around DNA in opposite orientations. MCM loading is orchestrated by binding of the origin recognition complex (ORC) to DNA, but how ORC coordinates symmetrical MCM loading is unclear. We used natural budding yeast DNA replication origins and synthetic DNA sequences to show that efficient MCM loading requires binding of two ORC molecules to two ORC binding sites. The relative orientation of these sites, but not the distance between them, was found to be critical for MCM loading in vitro and origin function in vivo. We propose that quasi-symmetrical loading of individual MCM hexamers by ORC and directed MCM translocation into double hexamers acts as a unifying mechanism for the establishment of bidirectional replication in archaea and eukaryotes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000405901600044 |
WOS关键词 | SACCHAROMYCES-CEREVISIAE ; MCM2-7 HELICASE ; ORIGINS ; COMPLEX ; ARCHITECTURE ; CHROMOSOME ; ACTIVATION ; INITIATION ; BINDING ; SITES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196510 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Francis Crick Inst, London NW1 1AT, England |
推荐引用方式 GB/T 7714 | Coster, Gideon,Diffley, John F. X.. Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading (vol 357, pg 314, 2017)[J]. SCIENCE,2017,357(6348):314-318. |
APA | Coster, Gideon,&Diffley, John F. X..(2017).Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading (vol 357, pg 314, 2017).SCIENCE,357(6348),314-318. |
MLA | Coster, Gideon,et al."Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading (vol 357, pg 314, 2017)".SCIENCE 357.6348(2017):314-318. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论