Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao0100 |
A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems | |
Kazlauskiene, Migle; Kostiuk, Georgij; Venclovas, Ceslovas; Tamulaitis, Gintautas; Siksnys, Virginijus | |
2017-08-11 | |
发表期刊 | SCIENCE
![]() |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6351页码:605-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Lithuania |
英文摘要 | Type III CRISPR-Cas systems in prokaryotes provide immunity against invading nucleic acids through the coordinated degradation of transcriptionally active DNA and its transcripts by the Csm effector complex. The Cas10 subunit of the complex contains an HD nuclease domain that is responsible for DNA degradation and two Palm domains with elusive functions. In addition, Csm6, a ribonuclease that is not part of the complex, is also required to provide full immunity. We show here that target RNA binding by the Csm effector complex of Streptococcus thermophilus triggers Cas10 to synthesize cyclic oligoadenylates (cA(n); n = 2 to 6) by means of the Palm domains. Acting as signaling molecules, cyclic oligoadenylates bind Csm6 to activate its nonspecific RNA degradation. This cyclic oligoadenylate-based signaling pathway coordinates different components of CRISPR-Cas to prevent phage infection and propagation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000407324800044 |
WOS关键词 | CRYSTAL-STRUCTURE ; EFFECTOR COMPLEX ; DNA CLEAVAGE ; RNA ; IMMUNITY ; PROTEIN ; RECOGNITION ; ENDORIBONUCLEASE ; SUPERFAMILY ; DEGRADATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196634 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Vilnius Univ, Inst Biotechnol, Sauletekio Ave 7, LT-10257 Vilnius, Lithuania |
推荐引用方式 GB/T 7714 | Kazlauskiene, Migle,Kostiuk, Georgij,Venclovas, Ceslovas,et al. A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems[J]. SCIENCE,2017,357(6351):605-+. |
APA | Kazlauskiene, Migle,Kostiuk, Georgij,Venclovas, Ceslovas,Tamulaitis, Gintautas,&Siksnys, Virginijus.(2017).A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems.SCIENCE,357(6351),605-+. |
MLA | Kazlauskiene, Migle,et al."A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems".SCIENCE 357.6351(2017):605-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论