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DOI | 10.1126/science.aan3351 |
Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex | |
Luo, Chongyuan1,2; Keown, Christopher L.3; Kurihara, Laurie4; Zhou, Jingtian1,5; He, Yupeng1,5; Li, Junhao3; Castanon, Rosa1; Lucero, Jacinta6; Nery, Joseph R.1; Sandoval, Justin P.1; Bui, Brian6; Sejnowski, Terrence J.2,6,7; Harkins, Timothy T.4; Mukamel, Eran A.3; Behrens, M. Margarita6; Ecker, Joseph R.1,2 | |
2017-08-11 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6351页码:600-604 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The mammalian brain contains diverse neuronal types, yet we lack single-cell epigenomic assays that are able to identify and characterize them. DNA methylation is a stable epigenetic mark that distinguishes cell types and marks regulatory elements. We generated >6000 methylomes from single neuronal nuclei and used them to identify 16 mouse and 21 human neuronal subpopulations in the frontal cortex. CG and non-CG methylation exhibited cell type-specific distributions, and we identified regulatory elements with differential methylation across neuron types. Methylation signatures identified a layer 6 excitatory neuron subtype and a unique human parvalbumin-expressing inhibitory neuron subtype. We observed stronger cross-species conservation of regulatory elements in inhibitory neurons than in excitatory neurons. Single-nucleus methylomes expand the atlas of brain cell types and identify regulatory elements that drive conserved brain cell diversity. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000407324800043 |
WOS关键词 | EPIGENETIC HETEROGENEITY ; EPIGENOMIC SIGNATURES ; RADIAL GLIA ; HUMAN BRAIN ; SPECIFICATION ; DIVERSITY ; GENOME |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196639 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Salk Inst Biol Studies, Genom Anal Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA; 2.Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA; 3.Univ Calif San Diego, Dept Cognit Sci, La Jolla, CA 92037 USA; 4.Swift Biosci Inc, 58 Parkland Plaza,Suite 100, Ann Arbor, MI 48103 USA; 5.Univ Calif San Diego, Bioinformat & Syst Biol Program, La Jolla, CA 92093 USA; 6.Salk Inst Biol Studies, Computat Neurobiol Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA; 7.Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Luo, Chongyuan,Keown, Christopher L.,Kurihara, Laurie,et al. Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex[J]. SCIENCE,2017,357(6351):600-604. |
APA | Luo, Chongyuan.,Keown, Christopher L..,Kurihara, Laurie.,Zhou, Jingtian.,He, Yupeng.,...&Ecker, Joseph R..(2017).Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex.SCIENCE,357(6351),600-604. |
MLA | Luo, Chongyuan,et al."Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex".SCIENCE 357.6351(2017):600-604. |
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