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DOI | 10.1126/science.aan4497 |
Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase | |
Shomura, Y.1; Taketa, M.2,3; Nakashima, H.2; Tai, H.3,4; Nakagawa, H.2; Ikeda, Y.2; Ishii, M.5; Igarashi, Y.5; Nishihara, H.6; Yoon, K-S.7,8; Ogo, S.7,8; Hirota, S.3,4; Higuchi, Y.2,3,9 | |
2017-09-01 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6354页码:928-931 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan |
英文摘要 | NAD(+) (oxidized form of NAD: nicotinamide adenine dinucleotide)-reducing soluble [ NiFe]hydrogenase (SH) is phylogenetically related to NADH (reduced form of NAD(+)): quinone oxidoreductase (complex I), but the geometrical arrangements of the subunits and Fe-S clusters are unclear. Here, we describe the crystal structures of SH in the oxidized and reduced states. The cluster arrangement is similar to that of complex I, but the subunits orientation is not, which supports the hypothesis that subunits evolved as prebuilt modules. The oxidized active site includes a six-coordinate Ni, which is unprecedented for hydrogenases, whose coordination geometry would prevent O-2 from approaching. In the reduced state showing the normal active site structure without a physiological electron acceptor, the flavin mononucleotide cofactor is dissociated, which may be caused by the oxidation state change of nearby Fe-S clusters and may suppress production of reactive oxygen species. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000408734900045 |
WOS关键词 | NAD-REDUCING HYDROGENASE ; RESPIRATORY COMPLEX-I ; ACTIVE-SITE ; RALSTONIA ; OPERON ; FTIR ; EPR ; FMN ; NICKEL ; STATE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196768 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Ibaraki Univ, Grad Sch Sci & Engn, Inst Quantum Beam Sci, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 3168511, Japan; 2.Univ Hyogo, Grad Sch Life Sci, Dept Picobiol, 3-2-1 Koto,Kamigori Cho, Kobe, Hyogo 6781297, Japan; 3.Japan & Sci Technol Agcy, CREST, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan; 4.Nara Inst Sci & Technol, Grad Sch Mat Sci, 8916-5 Takayama Cho, Ikoma, Nara 6300192, Japan; 5.Univ Tokyo, Dept Biotechnol, Grad Sch Agr & Life Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan; 6.Ibaraki Univ, Coll Agr, Dept Bioresource Sci, 3-21-1 Chu Ou, Ami, Ibaraki 3000393, Japan; 7.Kyushu Univ, World Premier Int Res Ctr Initiative, Int Inst Carbon Neutral Energy Res WPI I2CNER, Nishi Ku, 744 Moto Oka, Fukuoka 8190395, Japan; 8.Kyushu Univ, Dept Chem & Biochem, Grad Sch Engn, Nishi Ku, 744 Moto Oka, Fukuoka 8190395, Japan; 9.RIKEN, SPring Ctr 8, 1-1-1 Koto, Sayo, Hyogo 6795148, Japan |
推荐引用方式 GB/T 7714 | Shomura, Y.,Taketa, M.,Nakashima, H.,et al. Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase[J]. SCIENCE,2017,357(6354):928-931. |
APA | Shomura, Y..,Taketa, M..,Nakashima, H..,Tai, H..,Nakagawa, H..,...&Higuchi, Y..(2017).Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase.SCIENCE,357(6354),928-931. |
MLA | Shomura, Y.,et al."Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase".SCIENCE 357.6354(2017):928-931. |
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