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DOI | 10.1126/science.aan4574 |
Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase | |
McCarthy, Erin L.1; Booker, Squire J.1,2,3 | |
2017-10-20 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6361页码:373-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Lipoyl synthase (LipA) catalyzes the last step in the biosynthesis of the lipoyl cofactor, which is the attachment of two sulfhydryl groups to C6 and C8 of a pendant octanoyl chain. The appended sulfur atoms derive from an auxiliary [4Fe-4S] cluster on the protein that is degraded during turnover, limiting LipA to one turnover in vitro. We found that the Escherichia coli iron-sulfur (Fe-S) cluster carrier protein NfuA efficiently reconstitutes the auxiliary cluster during LipA catalysis in a step that is not rate-limiting. We also found evidence for a second pathway for cluster regeneration involving the E. coli protein IscU. These results show that enzymes that degrade their Fe-S clusters as a sulfur source can nonetheless act catalytically. Our results also explain why patients with NFU1 gene deletions exhibit phenotypes that are indicative of lipoyl cofactor deficiencies. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000413251000044 |
WOS关键词 | ADENOSYL-L-METHIONINE ; ESCHERICHIA-COLI ; 4FE-4S CLUSTERS ; ACID ; BIOSYNTHESIS ; ENZYMES ; PROTEIN ; NFUA ; INTERMEDIATE ; BIOGENESIS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197154 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA; 2.Penn State Univ, Dept Chem, University Pk, PA 16802 USA; 3.Penn State Univ, Howard Hughes Med Inst, University Pk, PA 16802 USA |
推荐引用方式 GB/T 7714 | McCarthy, Erin L.,Booker, Squire J.. Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase[J]. SCIENCE,2017,358(6361):373-+. |
APA | McCarthy, Erin L.,&Booker, Squire J..(2017).Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase.SCIENCE,358(6361),373-+. |
MLA | McCarthy, Erin L.,et al."Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase".SCIENCE 358.6361(2017):373-+. |
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