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DOI | 10.1126/science.aao1923 |
Structural basis of bacterial transcription activation | |
Liu, Bin1; Hong, Chuan2; Huang, Rick K.2; Yu, Zhiheng2; Steitz, Thomas A.1,3,4 | |
2017-11-17 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6365页码:947-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | In bacteria, the activation of gene transcription at many promoters is simple and only involves a single activator. The cyclic adenosine 3', 5'-monophosphate receptor protein (CAP), a classic activator, is able to activate transcription independently through two different mechanisms. Understanding the class I mechanism requires an intact transcription activation complex (TAC) structure at a high resolution. Here we report a high-resolution cryo-electron microscopy structure of an intact Escherichia coli class I TAC containing a CAP dimer, a sigma(70) -RNA polymerase (RNAP) holoenzyme, a complete class I CAP-dependent promoter DNA, and a de novo synthesized RNA oligonucleotide. The structure shows how CAP wraps the upstream DNA and how the interactions recruit RNAP. Our study provides a structural basis for understanding how activators activate transcription through the class I recruitment mechanism. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000415293000052 |
WOS关键词 | AMP RECEPTOR PROTEIN ; C-TERMINAL DOMAIN ; CAP-DNA COMPLEX ; COLI RNA-POLYMERASE ; STIMULATING TRANSCRIPTION ; INITIATION-COMPLEXES ; SUBUNIT ; IDENTIFICATION ; SIGMA(70) ; REGION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197344 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA; 2.Howard Hughes Med Inst, CryoEM Shared Resource, Janelia Res Campus, Ashburn, VA 20147 USA; 3.Yale Univ, Howard Hughes Med Inst, New Haven, CT 06510 USA; 4.Yale Univ, Dept Chem, New Haven, CT 06520 USA |
推荐引用方式 GB/T 7714 | Liu, Bin,Hong, Chuan,Huang, Rick K.,et al. Structural basis of bacterial transcription activation[J]. SCIENCE,2017,358(6365):947-+. |
APA | Liu, Bin,Hong, Chuan,Huang, Rick K.,Yu, Zhiheng,&Steitz, Thomas A..(2017).Structural basis of bacterial transcription activation.SCIENCE,358(6365),947-+. |
MLA | Liu, Bin,et al."Structural basis of bacterial transcription activation".SCIENCE 358.6365(2017):947-+. |
条目包含的文件 | 条目无相关文件。 |
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