Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao6535 ; 10.1126/science.aao6535 |
Architecture of eukaryotic mRNA 3 '-end processing machinery; Architecture of eukaryotic mRNA 3 '-end processing machinery | |
Casanal, Ana1; Kumar, Ananthanarayanan1; Hill, Chris H.1; Easter, Ashley D.1; Emsley, Paul1; Degliesposti, Gianluca1; Gordiyenko, Yuliya1,2; Santhanam, Balaji1; Wolf, Jana1; Wiederhold, Katrin1; Dornan, Gillian L.1; Skehel, Mark1; Robinson, Carol V.2; Passmore, Lori A.1 | |
2017-11-24 ; 2017-11-24 | |
发表期刊 | SCIENCE
![]() ![]() |
ISSN | 0036-8075 ; 0036-8075 |
EISSN | 1095-9203 ; 1095-9203 |
出版年 | 2017 ; 2017 |
卷号 | 358期号:6366页码:1056-1059 |
文章类型 | Article ; Article |
语种 | 英语 ; 英语 |
国家 | England; England |
英文摘要 | Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the similar to 1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the similar to 200-kilodalton polymerase module. This revealed four beta propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3 '-end processing. ; Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the similar to 1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the similar to 200-kilodalton polymerase module. This revealed four beta propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3 '-end processing. |
领域 | 地球科学 ; 地球科学 ; 气候变化 ; 气候变化 ; 资源环境 ; 资源环境 |
收录类别 | SCI-E ; SCI-E |
WOS记录号 | WOS:000416590400040 ; WOS:000416590400040 |
WOS关键词 | POLYADENYLATION SPECIFICITY FACTOR ; POLYADENYLATION SPECIFICITY FACTOR ; YEAST POLY(A) POLYMERASE ; YEAST POLY(A) POLYMERASE ; C-TERMINAL-DOMAIN ; C-TERMINAL-DOMAIN ; SACCHAROMYCES-CEREVISIAE ; SACCHAROMYCES-CEREVISIAE ; CLEAVAGE/POLYADENYLATION FACTOR ; CLEAVAGE/POLYADENYLATION FACTOR ; STRUCTURAL BASIS ; STRUCTURAL BASIS ; 3-END FORMATION ; 3-END FORMATION ; CLEAVAGE ; CLEAVAGE ; PROTEIN ; PROTEIN ; COMPLEX ; COMPLEX |
WOS类目 | Multidisciplinary Sciences ; Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics ; Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197388 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.MRC, Lab Mol Biol, Cambridge, England; 2.Univ Oxford, Chem Res Lab, Oxford, England |
推荐引用方式 GB/T 7714 | Casanal, Ana,Kumar, Ananthanarayanan,Hill, Chris H.,et al. Architecture of eukaryotic mRNA 3 '-end processing machinery, Architecture of eukaryotic mRNA 3 '-end processing machinery[J]. SCIENCE, SCIENCE,2017, 2017,358, 358(6366):1056-1059, 1056-1059. |
APA | Casanal, Ana.,Kumar, Ananthanarayanan.,Hill, Chris H..,Easter, Ashley D..,Emsley, Paul.,...&Passmore, Lori A..(2017).Architecture of eukaryotic mRNA 3 '-end processing machinery.SCIENCE,358(6366),1056-1059. |
MLA | Casanal, Ana,et al."Architecture of eukaryotic mRNA 3 '-end processing machinery".SCIENCE 358.6366(2017):1056-1059. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论