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DOI | 10.1126/science.aar3729 |
Postcatalytic spliceosome structure reveals mechanism of 3 '-splice site selection | |
Wilkinson, Max E.1; Fica, Sebastian M.1; Galej, Wojciech P.1,2; Norman, Christine M.1; Newman, Andrew J.1; Nagai, Kiyoshi1 | |
2017-12-08 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6368页码:1283-1287 |
文章类型 | Article |
语种 | 英语 |
国家 | England; France |
英文摘要 | Introns are removed from eukaryotic messenger RNA precursors by the spliceosome in two transesterification reactions-branching and exon ligation. The mechanism of 3'-splice site recognition during exon ligation has remained unclear. Here we present the 3.7-angstrom cryo-electron microscopy structure of the yeast P-complex spliceosome immediately after exon ligation. The 3'-splice site AG dinucleotide is recognized through non-Watson-Crick pairing with the 5' splice site and the branch-point adenosine. After the branching reaction, protein factors work together to remodel the spliceosome and stabilize a conformation competent for 3'-splice site docking, thereby promoting exon ligation. The structure accounts for the strict conservation of the GU and AG dinucleotides at the 5' and 3' ends of introns and provides insight into the catalytic mechanism of exon ligation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000417254700054 |
WOS关键词 | PRE-MESSENGER-RNA ; CRYO-EM STRUCTURE ; SACCHAROMYCES-CEREVISIAE ; ACTIVATED SPLICEOSOME ; CRYSTAL-STRUCTURE ; CATALYTIC STEPS ; 2ND STEP ; ANGSTROM RESOLUTION ; U6 SNRNA ; U5 SNRNA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197475 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.MRC Lab Mol Biol, Francis Crick Ave, Cambridge CB2 0QH, England; 2.European Mol Biol Lab EMBL Grenoble, 71 Ave Martyrs, F-38042 Grenoble 09, France |
推荐引用方式 GB/T 7714 | Wilkinson, Max E.,Fica, Sebastian M.,Galej, Wojciech P.,et al. Postcatalytic spliceosome structure reveals mechanism of 3 '-splice site selection[J]. SCIENCE,2017,358(6368):1283-1287. |
APA | Wilkinson, Max E.,Fica, Sebastian M.,Galej, Wojciech P.,Norman, Christine M.,Newman, Andrew J.,&Nagai, Kiyoshi.(2017).Postcatalytic spliceosome structure reveals mechanism of 3 '-splice site selection.SCIENCE,358(6368),1283-1287. |
MLA | Wilkinson, Max E.,et al."Postcatalytic spliceosome structure reveals mechanism of 3 '-splice site selection".SCIENCE 358.6368(2017):1283-1287. |
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