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DOI | 10.1126/science.aao3435 |
Structural basis for methylphosphonate biosynthesis | |
Born, David A.1,2; Ulrich, Emily C.3,4; Ju, Kou-San4,5,6; Peck, Spencer C.3,4,10; van der Donk, Wilfred A.3,4,7; Drennan, Catherine L.2,8,9 | |
2017-12-08 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6368页码:1336-1338 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Methylphosphonate synthase (MPnS) produces methylphosphonate, a metabolic precursor to methane in the upper ocean. Here, we determine a 2.35-angstrom resolution structure of MPnS and discover that it has an unusual 2-histidine-1-glutamine iron-coordinating triad. We further solve the structure of a related enzyme, hydroxyethylphosphonate dioxygenase from Streptomyces albus (SaHEPD), and find that it displays the same motif. SaHEPD can be converted into an MPnS by mutation of glutamine-adjacent residues, identifying the molecular requirements for methylphosphonate synthesis. Using these sequence markers, we find numerous putative MPnSs in marine microbiomes and confirm that MPnS is present in the abundant Pelagibacter ubique. The ubiquity of MPnS-containing microbes supports the proposal that methylphosphonate is a source of methane in the upper, aerobic ocean, where phosphorus-starved microbes catabolize methylphosphonate for its phosphorus. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000417254700066 |
WOS关键词 | METHANE ; CLEAVAGE ; OCEAN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197490 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Grad Program Biophys, Cambridge, MA 02138 USA; 2.MIT, Dept Biol, Cambridge, MA USA; 3.Univ Illinois, Dept Chem, Urbana, IL USA; 4.Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL USA; 5.Ohio State Univ, Dept Microbiol, 484 W 12th Ave, Columbus, OH 43210 USA; 6.Ohio State Univ, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USA; 7.Univ Illinois, Howard Hughes Med Inst, Urbana, IL USA; 8.MIT, Dept Chem, Cambridge, MA 02139 USA; 9.MIT, Howard Hughes Med Inst, Cambridge, MA USA; 10.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA |
推荐引用方式 GB/T 7714 | Born, David A.,Ulrich, Emily C.,Ju, Kou-San,et al. Structural basis for methylphosphonate biosynthesis[J]. SCIENCE,2017,358(6368):1336-1338. |
APA | Born, David A.,Ulrich, Emily C.,Ju, Kou-San,Peck, Spencer C.,van der Donk, Wilfred A.,&Drennan, Catherine L..(2017).Structural basis for methylphosphonate biosynthesis.SCIENCE,358(6368),1336-1338. |
MLA | Born, David A.,et al."Structural basis for methylphosphonate biosynthesis".SCIENCE 358.6368(2017):1336-1338. |
条目包含的文件 | 条目无相关文件。 |
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