Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao0958 |
Multiplex recording of cellular events over time on CRISPR biological tape | |
Sheth, Ravi U.1,2; Yim, Sung Sun1; Wu, Felix L.1,2; Wang, Harris H.1,3 | |
2017-12-15 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6369页码:1457-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Although dynamics underlie many biological processes, our ability to robustly and accurately profile time-varying biological signals and regulatory programs remains limited. Here we describe a framework for storing temporal biological information directly in the genomes of a cell population. We developed a "biological tape recorder" in which biological signals trigger intracellular DNA production that is then recorded by the CRISPR-Cas adaptation system. This approach enables stable recording over multiple days and accurate reconstruction of temporal and lineage information by sequencing CRISPR arrays. We further demonstrate a multiplexing strategy to simultaneously record the temporal availability of three metabolites (copper, trehalose, and fucose) in the environment of a cell population over time. This work enables the temporal measurement of dynamic cellular states and environmental changes and suggests new applications for chronicling biological events on a large scale. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000417918500047 |
WOS关键词 | IMMUNE-RESPONSE ; LIVING BACTERIA ; CAS SYSTEMS ; ACQUISITION ; ADAPTATION ; GENOME ; CELLS ; DNA ; PROKARYOTES ; INTEGRATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197561 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Columbia Univ, Dept Syst Biol, New York, NY 10027 USA; 2.Columbia Univ, Integrated Program Cellular Mol & Biomed Studies, New York, NY USA; 3.Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10027 USA |
推荐引用方式 GB/T 7714 | Sheth, Ravi U.,Yim, Sung Sun,Wu, Felix L.,et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. SCIENCE,2017,358(6369):1457-+. |
APA | Sheth, Ravi U.,Yim, Sung Sun,Wu, Felix L.,&Wang, Harris H..(2017).Multiplex recording of cellular events over time on CRISPR biological tape.SCIENCE,358(6369),1457-+. |
MLA | Sheth, Ravi U.,et al."Multiplex recording of cellular events over time on CRISPR biological tape".SCIENCE 358.6369(2017):1457-+. |
条目包含的文件 | 条目无相关文件。 |
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