Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao4277 |
Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq | |
Medaglia, Chiara1; Giladi, Amir1; Stoler-Barak, Liat1; De Giovanni, Marco2,3,4; Salame, Tomer Meir5; Biram, Adi1; David, Eyal1; Li, Hanjie1; Iannacone, Matteo2,3,4; Shulman, Ziv1; Amit, Ido1 | |
2017-12-22 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6370页码:1622-1626 |
文章类型 | Article |
语种 | 英语 |
国家 | Israel; Italy |
英文摘要 | Cellular functions are strongly dependent on surrounding cells and environmental factors. Current technologies are limited in their ability to characterize the spatial location and gene programs of cells in poorly structured and dynamic niches. We developed a method, NICHE-seq, that combines photoactivatable fluorescent reporters, two-photon microscopy, and single-cell RNA sequencing (scRNA-seq) to infer the cellular and molecular composition of niches. We applied NICHE-seq to examine the high-order assembly of immune cell networks. NICHE-seq is highly reproducible in spatial tissue reconstruction, enabling identification of rare niche-specific immune subpopulations and gene programs, including natural killer cells within infected B cell follicles and distinct myeloid states in the spleen and tumor. This study establishes NICHE-seq as a broadly applicable method for elucidating high-order spatial organization of cell types and their molecular pathways. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000418448000067 |
WOS关键词 | GENE-EXPRESSION ; SINGLE CELLS ; HETEROGENEITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197608 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Weizmann Inst Sci, Dept Immunol, Rehovot, Israel; 2.Ist Sci San Raffaele, IRCCS, Div Immunol Transplantat & Infect Dis, I-20132 Milan, Italy; 3.Ist Sci San Raffaele, IRCCS, Expt Imaging Ctr, I-20132 Milan, Italy; 4.Univ Vita Salute San Raffaele, I-20132 Milan, Italy; 5.Weizmann Inst Sci, Dept Biol Serv, Flow Cytometry Unit, Rehovot, Israel |
推荐引用方式 GB/T 7714 | Medaglia, Chiara,Giladi, Amir,Stoler-Barak, Liat,et al. Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq[J]. SCIENCE,2017,358(6370):1622-1626. |
APA | Medaglia, Chiara.,Giladi, Amir.,Stoler-Barak, Liat.,De Giovanni, Marco.,Salame, Tomer Meir.,...&Amit, Ido.(2017).Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq.SCIENCE,358(6370),1622-1626. |
MLA | Medaglia, Chiara,et al."Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq".SCIENCE 358.6370(2017):1622-1626. |
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