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DOI | 10.1126/science.aar2781 |
Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N | |
Chittori, Sagar1; Hong, Jingjun2; Saunders, Hayden2; Feng, Hanqiao2; Ghirlando, Rodolfo3; Kelly, Alexander E.2; Bai, Yawen2; Subramaniam, Sriram1 | |
2018-01-19 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 359期号:6373页码:339-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Accurate chromosome segregation requires the proper assembly of kinetochore proteins. A key step in this process is the recognition of the histone H3 variant CENP-A in the centromeric nucleosome by the kinetochore protein CENP-N. We report cryo-electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studies of the interaction between the CENP-A nucleosome and CENP-N. We show that human CENP-N confers binding specificity through interactions with the L1 loop of CENP-A, stabilized by electrostatic interactions with the nucleosomal DNA. Mutational analyses demonstrate analogous interactions in Xenopus, which are further supported by residue-swapping experiments involving the L1 loop of CENP-A. Our results are consistent with the coevolution of CENP-N and CENP-A and establish the structural basis for recognition of the CENP-A nucleosome to enable kinetochore assembly and centromeric chromatin organization. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000423236700041 |
WOS关键词 | A NUCLEOSOMES ; CHROMATIN ; HISTONE ; ORGANIZATION ; ARCHITECTURE ; HETERODIMER ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197799 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.NCI, Lab Cell Biol, CCR, NIH, Bethesda, MD 20892 USA; 2.NCI, Lab Biochem & Mol Biol, CCR, NIH, Bethesda, MD 20892 USA; 3.NIDDK, Lab Mol Biol, NIH, Bethesda, MD 20892 USA |
推荐引用方式 GB/T 7714 | Chittori, Sagar,Hong, Jingjun,Saunders, Hayden,et al. Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N[J]. SCIENCE,2018,359(6373):339-+. |
APA | Chittori, Sagar.,Hong, Jingjun.,Saunders, Hayden.,Feng, Hanqiao.,Ghirlando, Rodolfo.,...&Subramaniam, Sriram.(2018).Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N.SCIENCE,359(6373),339-+. |
MLA | Chittori, Sagar,et al."Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N".SCIENCE 359.6373(2018):339-+. |
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