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DOI | 10.1126/science.aao6135 |
A pathway for mitotic chromosome formation | |
Gibcus, Johan H.1; Samejima, Kumiko2; Goloborodko, Anton3,4; Samejima, Itaru2; Naumova, Natalia1; Nuebler, Johannes3,4; Kanemaki, Masato T.5,6; Xie, Linfeng7; Paulson, James R.7; Earnshaw, William C.2; Mirny, Leonid A.3,4; Dekker, Job1,8 | |
2018-02-09 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 359期号:6376页码:652-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Scotland; Japan |
英文摘要 | Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, similar to 80-kb inner loops are nested within similar to 400-kb outer loops. The loop array acquires a helical arrangement with consecutive loops emanating from a central "spiral staircase" condensin scaffold. The size of helical turns progressively increases to similar to 12 megabases during prometaphase. Acute depletion of condensin I or II shows that nested loops form by differential action of the two condensins, whereas condensin II is required for helical winding. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000438784100002 |
WOS关键词 | SISTER-CHROMATID RESOLUTION ; CONDENSIN-I ; METAPHASE CHROMOSOMES ; VERTEBRATE CELLS ; TOPOISOMERASE-II ; SCAFFOLD ATTACHMENT ; CHROMOKINESIN KIF4A ; MAMMALIAN-CELLS ; LOOP EXTRUSION ; HUMAN GENOME |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197949 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Massachusetts, Dept Biochem & Mol Pharmacol, Program Syst Biol, Med Sch, 368 Plantation St, Worcester, MA 01605 USA; 2.Univ Edinburgh, Wellcome Ctr Cell Biol, Kings Bldg, Edinburgh EH9 3BF, Midlothian, Scotland; 3.MIT, Inst Med Engn & Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 4.MIT, Dept Phys, Cambridge, MA 02139 USA; 5.Res Org Informat & Syst, Div Mol Cell Engn, Natl Inst Genet, Yata 1111, Mishima, Shizuoka 4118540, Japan; 6.SOKENDAI, Dept Genet, Mishima, Shizuoka 4118540, Japan; 7.Univ Wisconsin, Dept Chem, 800 Algoma Blvd, Oshkosh, WI 54901 USA; 8.Univ Massachusetts, Howard Hughes Med Inst, Med Sch, 368 Plantation St, Worcester, MA 01605 USA |
推荐引用方式 GB/T 7714 | Gibcus, Johan H.,Samejima, Kumiko,Goloborodko, Anton,et al. A pathway for mitotic chromosome formation[J]. SCIENCE,2018,359(6376):652-+. |
APA | Gibcus, Johan H..,Samejima, Kumiko.,Goloborodko, Anton.,Samejima, Itaru.,Naumova, Natalia.,...&Dekker, Job.(2018).A pathway for mitotic chromosome formation.SCIENCE,359(6376),652-+. |
MLA | Gibcus, Johan H.,et al."A pathway for mitotic chromosome formation".SCIENCE 359.6376(2018):652-+. |
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