GSTDTAP  > 地球科学
DOI10.1126/science.aar7831
Real-time imaging of DNA loop extrusion by condensin
Ganji, Mahipal1; Shaltiel, Indra A.2; Bisht, Shveta2; Kim, Eugene1; Kalichava, Ana1; Haering, Christian H.2; Dekker, Cees1
2018-04-06
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2018
卷号360期号:6384页码:102-105
文章类型Article
语种英语
国家Netherlands; Germany
英文摘要

It has been hypothesized that SMC protein complexes such as condensin and cohesin spatially organize chromosomes by extruding DNA into large loops. We directly visualized the formation and processive extension of DNA loops by yeast condensin in real time. Our findings constitute unambiguous evidence for loop extrusion. We observed that a single condensin complex is able to extrude tens of kilobase pairs of DNA at a force-dependent speed of up to 1500 base pairs per second, using the energy of adenosine triphosphate hydrolysis. Condensin-induced loop extrusion was strictly asymmetric, which demonstrates that condensin anchors onto DNA and reels it in from only one side. Active DNA loop extrusion by SMC complexes may provide the universal unifying principle for genome organization.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000429263100055
WOS关键词SINGLE-MOLECULE ; SMC COMPLEXES ; CHROMOSOME ; MECHANISM ; ORGANIZATION ; COMPACTION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/198335
专题地球科学
资源环境科学
气候变化
作者单位1.Delft Univ Technol, Kavli Inst Nanosci Delft, Dept Bionanosci, Delft, Netherlands;
2.European Mol Biol Lab, Struct & Computat Biol Unit, Cell Biol & Biophys Unit, Heidelberg, Germany
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GB/T 7714
Ganji, Mahipal,Shaltiel, Indra A.,Bisht, Shveta,et al. Real-time imaging of DNA loop extrusion by condensin[J]. SCIENCE,2018,360(6384):102-105.
APA Ganji, Mahipal.,Shaltiel, Indra A..,Bisht, Shveta.,Kim, Eugene.,Kalichava, Ana.,...&Dekker, Cees.(2018).Real-time imaging of DNA loop extrusion by condensin.SCIENCE,360(6384),102-105.
MLA Ganji, Mahipal,et al."Real-time imaging of DNA loop extrusion by condensin".SCIENCE 360.6384(2018):102-105.
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