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DOI | 10.1126/science.aap8992 |
Rewritable multi-event analog recording in bacterial and mammalian cells | |
Tang, Weixin; Liu, David R.1 | |
2018-04-13 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6385 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | We present two CRISPR-mediated analog multi-event recording apparatus (CAMERA) systems that use base editors and Cas9 nucleases to record cellular events in bacteria and mammalian cells. The devices record signal amplitude or duration as changes in the ratio of mutually exclusive DNA sequences (CAMERA 1) or as single-base modifications (CAMERA 2). We achieved recording of multiple stimuli in bacteria or mammalian cells, including exposure to antibiotics, nutrients, viruses, light, and changes in Wnt signaling. When recording to multicopy plasmids, reliable readout requires as few as 10 to 100 cells. The order of stimuli can be recorded through an overlapping guide RNA design, and memories can be erased and re-recorded over multiple cycles. CAMERA systems serve as "cell data recorders" that write a history of endogenous or exogenous signaling events into permanent DNA sequence modifications in living cells. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000429805400037 |
WOS关键词 | MOLECULAR-MECHANISM ; GENOMIC DNA ; BASE ; MEMORY ; ACTIVATION ; EXPRESSION ; CIRCUITS ; LOGIC ; RICE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
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文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198408 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Broad Inst MIT & Harvard, Merkin Inst Transformat Technol Healthcare, Cambridge, MA 02142 USA; 2.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 3.Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA |
推荐引用方式 GB/T 7714 | Tang, Weixin,Liu, David R.. Rewritable multi-event analog recording in bacterial and mammalian cells[J]. SCIENCE,2018,360(6385). |
APA | Tang, Weixin,&Liu, David R..(2018).Rewritable multi-event analog recording in bacterial and mammalian cells.SCIENCE,360(6385). |
MLA | Tang, Weixin,et al."Rewritable multi-event analog recording in bacterial and mammalian cells".SCIENCE 360.6385(2018). |
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