Field-deployable viral diagnostics using CRISPR-Cas13

doi:10.1126/science.aas8836

GSTDTAP > 地球科学

DOI	10.1126/science.aas8836
	Field-deployable viral diagnostics using CRISPR-Cas13
	Myhrvold, Cameron 1,2; Freije, Catherine A.1,2,3; Gootenberg, Jonathan S.1,4,5,6,7; Abudayyeh, Omar O.1,5,6,7,8; Metsky, Hayden C.1,9; Durbin, Ann F.3,10; Kellner, Max J.1; Tan, Amanda L.11; Paul, Lauren M.11; Parham, Leda A.12; Garcia, Kimberly F.12; Barnes, Kayla G.1,2,13; Chak, Bridget 1,2; Mondini, Adriano 14; Nogueira, Mauricio L.15; Isern, Sharon 11; Michael, Scott F.11; Lorenzana, Ivette 12; Yozwiak, Nathan L.1,2; MacInnis, Bronwyn L.1,13; Bosch, Irene 10,16; Gehrke, Lee 3,10,17; Zhang, Feng 1,2,5,6,7; Sabeti, Pardis C.1,2,3,13,18
	2018-04-27
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2018
卷号	360 期号:6387 页码:444-448
文章类型	Article
语种	英语
国家	USA; Honduras; Brazil
英文摘要	Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENVdetection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015-2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000430949600044
WOS关键词	ZIKA-VIRUS ; PCR ; AMPLIFICATION ; INFECTION ; AMERICA ; ASSAY
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/198503
专题	地球科学资源环境科学气候变化
作者单位	1.Broad Inst Massachusetts Inst Technol MIT & Harva, Cambridge, MA 02142 USA; 2.Harvard Univ, Dept Organismal & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 USA; 3.Harvard Med Sch, Div Med Sci, PhD Program Virol, Boston, MA 02115 USA; 4.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA; 5.MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA; 6.MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA; 7.MIT, Dept Biol Engn, Cambridge, MA 02139 USA; 8.MIT, Dept Hlth Sci & Technol, Cambridge, MA 02139 USA; 9.MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA; 10.MIT, Inst Med Engn & Sci, Cambridge, MA 02139 USA; 11.Florida Gulf Coast Univ, Dept Biol Sci, Ft Myers, FL 33965 USA; 12.Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Ctr Invest Genet, Tegucigalpa, Honduras; 13.Harvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA; 14.Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara Lab Publ Hlth, Sao Paulo, Brazil; 15.Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Paulo, Brazil; 16.Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA; 17.Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 USA; 18.HHMI, Chevy Chase, MD 20815 USA
推荐引用方式 GB/T 7714	Myhrvold, Cameron,Freije, Catherine A.,Gootenberg, Jonathan S.,et al. Field-deployable viral diagnostics using CRISPR-Cas13[J]. SCIENCE,2018,360(6387):444-448.
APA	Myhrvold, Cameron.,Freije, Catherine A..,Gootenberg, Jonathan S..,Abudayyeh, Omar O..,Metsky, Hayden C..,...&Sabeti, Pardis C..(2018).Field-deployable viral diagnostics using CRISPR-Cas13.SCIENCE,360(6387),444-448.
MLA	Myhrvold, Cameron,et al."Field-deployable viral diagnostics using CRISPR-Cas13".SCIENCE 360.6387(2018):444-448.