Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao0645 |
Morphogen gradient reconstitution reveals Hedgehog pathway design principles | |
Li, Pulin1; Markson, Joseph S.1; Wang, Sheng1; Chen, Siheng1; Vachharajani, Vipul1; Elowitz, Michael B.1,2,3 | |
2018-05-04 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6388页码:543-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | In developing tissues, cells estimate their spatial position by sensing graded concentrations of diffusible signaling proteins called morphogens. Morphogen-sensing pathways exhibit diverse molecular architectures, whose roles in controlling patterning dynamics and precision have been unclear. In this work, combining cell-based in vitro gradient reconstitution, genetic rewiring, and mathematical modeling, we systematically analyzed the distinctive architectural features of the Sonic Hedgehog pathway. We found that the combination of double-negative regulatory logic and negative feedback through the PTCH receptor accelerates gradient formation and improves robustness to variation in the morphogen production rate compared with alternative designs. The ability to isolate morphogen patterning from concurrent developmental processes and to compare the patterning behaviors of alternative, rewired pathway architectures offers a powerful way to understand and engineer multicellular patterning. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431351500048 |
WOS关键词 | SONIC HEDGEHOG ; NEURAL-TUBE ; RANGE ; GENE ; MECHANISMS ; PATTERN ; ROLES ; FORM ; SHH |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198581 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA; 2.CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA; 3.CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA |
推荐引用方式 GB/T 7714 | Li, Pulin,Markson, Joseph S.,Wang, Sheng,et al. Morphogen gradient reconstitution reveals Hedgehog pathway design principles[J]. SCIENCE,2018,360(6388):543-+. |
APA | Li, Pulin,Markson, Joseph S.,Wang, Sheng,Chen, Siheng,Vachharajani, Vipul,&Elowitz, Michael B..(2018).Morphogen gradient reconstitution reveals Hedgehog pathway design principles.SCIENCE,360(6388),543-+. |
MLA | Li, Pulin,et al."Morphogen gradient reconstitution reveals Hedgehog pathway design principles".SCIENCE 360.6388(2018):543-+. |
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