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DOI | 10.1126/science.aas9699 |
High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane | |
Srivastava, Anurag P.1; Luo, Min2; Zhou, Wenchang3; Symersky, Jindrich1; Bai, Dongyang1; Chambers, Melissa G.2; Faraldo-Gomez, Jose D.3; Liao, Maofu2; Mueller, David M.1 | |
2018-05-11 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6389 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Mitochondrial adenosine triphosphate (ATP) synthase comprises a membrane embedded F-o motor that rotates to drive ATP synthesis in the F-1 subunit. We used single-particle cryo-electron microscopy (cryo-EM) to obtain structures of the full complex in a lipid bilayer in the absence or presence of the inhibitor oligomycin at 3.6-and 3.8-angstrom resolution, respectively. To limit conformational heterogeneity, we locked the rotor in a single conformation by fusing the F6 subunit of the stator with the delta subunit of the rotor. Assembly of the enzyme with the F6-delta fusion caused a twisting of the rotor and a 9 degrees rotation of the F-o c(10)-ring in the direction of ATP synthesis, relative to the structure of isolated F-o. Our cryo-EM structures show how F-1 and F-o are coupled, give insight into the proton translocation pathway, and show how oligomycin blocks ATP synthesis. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431790900035 |
WOS关键词 | PROTON TRANSLOCATION ; STRUCTURAL BASIS ; BINDING-SITE ; FORCE-FIELD ; MECHANISM ; SUBUNIT ; MODEL ; VALIDATION ; RING ; ARRANGEMENT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198618 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Rosalind Franklin Univ, Dept Biol Chem & Mol Biol, Chicago Med Sch, 3333 Green Bay Rd, N Chicago, IL 60064 USA; 2.Harvard Med Sch, Dept Cell Biol, 250 Longwood Ave,SGM 509, Boston, MA 02115 USA; 3.NHLBI, Theoret Mol Biophys Lab, NIH, 50 South Dr, Bethesda, MD 20892 USA |
推荐引用方式 GB/T 7714 | Srivastava, Anurag P.,Luo, Min,Zhou, Wenchang,et al. High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane[J]. SCIENCE,2018,360(6389). |
APA | Srivastava, Anurag P..,Luo, Min.,Zhou, Wenchang.,Symersky, Jindrich.,Bai, Dongyang.,...&Mueller, David M..(2018).High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane.SCIENCE,360(6389). |
MLA | Srivastava, Anurag P.,et al."High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane".SCIENCE 360.6389(2018). |
条目包含的文件 | 条目无相关文件。 |
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