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DOI | 10.1126/science.aat0839 |
Structure basis for RNA-guided DNA degradation by Cascade and Cas3 | |
Xiao, Yibei1,3; Luo, Min2; Dolan, Adam E.1; Liao, Maofu2; Ke, Ailong1 | |
2018-07-06 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6397页码:41-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA by the RNA-guided Cascade (CRISPR associated complex for antiviral defense) complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here, we present a 3.7-angstrom-resolution cryo-electron microscopy (cryo-EM) structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop-forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the nontarget strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7-angstrom-resolution cryo-EM structure captures the postnicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for adenosine 5'-triphosphate-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000437467100055 |
WOS关键词 | IN-VITRO RECONSTITUTION ; SURVEILLANCE COMPLEX ; ESCHERICHIA-COLI ; IMMUNE-SYSTEM ; CRYSTAL-STRUCTURE ; CRISPR IMMUNITY ; FOREIGN DNA ; REPEATS ; TARGET ; INTERFERENCE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199076 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Cornell Univ, Dept Mol Biol & Genet, 253 Biotechnol Bldg, Ithaca, NY 14853 USA; 2.Harvard Med Sch, Dept Cell Biol, 250 Longwood Ave,SGM 509, Boston, MA 02115 USA; 3.China Pharmaceut Univ, State Key Lab Nat Med, Dept Pharmacology, Nanjing 210009, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Xiao, Yibei,Luo, Min,Dolan, Adam E.,et al. Structure basis for RNA-guided DNA degradation by Cascade and Cas3[J]. SCIENCE,2018,361(6397):41-+. |
APA | Xiao, Yibei,Luo, Min,Dolan, Adam E.,Liao, Maofu,&Ke, Ailong.(2018).Structure basis for RNA-guided DNA degradation by Cascade and Cas3.SCIENCE,361(6397),41-+. |
MLA | Xiao, Yibei,et al."Structure basis for RNA-guided DNA degradation by Cascade and Cas3".SCIENCE 361.6397(2018):41-+. |
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