Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aar7042 |
Multiplexed protein maps link subcellular organization to cellular states | |
Gut, Gabriele1,2; Herrmann, Markus D.1,3; Pelkmans, Lucas1 | |
2018-08-03 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6401 |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland |
英文摘要 | Obtaining highly multiplexed protein measurements across multiple length scales has enormous potential for biomedicine. Here, we measured, by iterative indirect immunofluorescence imaging (4i), 40-plex protein readouts from biological samples at high-throughput from the millimeter to the nanometer scale. This approach simultaneously captures properties apparent at the population, cellular, and subcellular levels, including microenvironment, cell shape, and cell cycle state. It also captures the detailed morphology of organelles, cytoskeletal structures, nuclear subcompartments, and the fate of signaling receptors in thousands of single cells in situ. We used computer vision and systems biology approaches to achieve unsupervised comprehensive quantification of protein subcompartmentalization within various multicellular, cellular, and pharmacological contexts. Thus, highly multiplexed subcellular protein maps can be used to identify functionally relevant single-cell states. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000440627300033 |
WOS关键词 | SINGLET-OXYGEN ; INACTIVATION ; ENDOCYTOSIS ; VARIABILITY ; REACTIVITY ; ANTIBODIES ; CELLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199294 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Zurich, Inst Mol Life Sci, Zurich, Switzerland; 2.Univ Zurich, Life Sci Zurich Grad Sch, Mol Life Sci PhD Program, Zurich, Switzerland; 3.Univ Zurich, Life Sci Zurich Grad Sch, MD PhD & Syst Biol PhD Program, Zurich, Switzerland |
推荐引用方式 GB/T 7714 | Gut, Gabriele,Herrmann, Markus D.,Pelkmans, Lucas. Multiplexed protein maps link subcellular organization to cellular states[J]. SCIENCE,2018,361(6401). |
APA | Gut, Gabriele,Herrmann, Markus D.,&Pelkmans, Lucas.(2018).Multiplexed protein maps link subcellular organization to cellular states.SCIENCE,361(6401). |
MLA | Gut, Gabriele,et al."Multiplexed protein maps link subcellular organization to cellular states".SCIENCE 361.6401(2018). |
条目包含的文件 | 条目无相关文件。 |
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