Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat1022 |
DNA-induced liquid phase condensation of cGAS activates innate immune signaling | |
Du, Mingjian1,2; Chen, Zhijian J.1,2,3 | |
2018-08-17 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6403页码:704-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The binding of DNA to cyclic GMP-AMP synthase (cGAS) leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP), which activates innate immune responses. We have shown that DNA binding to cGAS robustly induced the formation of liquidlike droplets in which cGAS was activated. The disordered and positively charged cGAS N terminus enhanced cGAS-DNA phase separation by increasing the valencies of DNA binding. Long DNA was more efficient in promoting cGAS liquid phase separation and cGAS enzyme activity than short DNA. Moreover, free zinc ions enhanced cGAS enzyme activity both in vitro and in cells by promoting cGAS-DNA phase separation. These results demonstrated that the DNA-induced phase transition of cGAS promotes cGAMP production and innate immune signaling. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000442818200045 |
WOS关键词 | CYCLIC GMP-AMP ; CELL-FREE FORMATION ; CYTOSOLIC DNA ; 2ND-MESSENGER ; GRANULES ; SYNTHASE ; TRANSITIONS ; PROTEINS ; DROPLETS ; PATHWAY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199381 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA; 2.Univ Texas Southwestern Med Ctr Dallas, Ctr Inflammat Res, Dallas, TX 75390 USA; 3.Univ Texas Southwestern Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA |
推荐引用方式 GB/T 7714 | Du, Mingjian,Chen, Zhijian J.. DNA-induced liquid phase condensation of cGAS activates innate immune signaling[J]. SCIENCE,2018,361(6403):704-+. |
APA | Du, Mingjian,&Chen, Zhijian J..(2018).DNA-induced liquid phase condensation of cGAS activates innate immune signaling.SCIENCE,361(6403),704-+. |
MLA | Du, Mingjian,et al."DNA-induced liquid phase condensation of cGAS activates innate immune signaling".SCIENCE 361.6403(2018):704-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论