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DOI | 10.1126/science.aau1504 |
Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation | |
Vemu, Annapurna1; Szczesna, Ewa1; Zehr, Elena A.1; Spector, Jeffrey O.1; Grigorieff, Nikolaus2,5; Deaconescu, Alexandra M.3; Roll-Mecak, Antonina1,4 | |
2018-08-24 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6404 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Spastin and katanin sever and destabilize microtubules. Paradoxically, despite their destructive activity they increase microtubule mass in vivo. We combined single-molecule total internal reflection fluorescence microscopy and electron microscopy to show that the elemental step in microtubule severing is the generation of nanoscale damage throughout the microtubule by active extraction of tubulin heterodimers. These damage sites are repaired spontaneously by guanosine triphosphate (GTP)-tubulin incorporation, which rejuvenates and stabilizes the microtubule shaft. Consequently, spastin and katanin increase microtubule rescue rates. Furthermore, newly severed ends emerge with a high density of GTP-tubulin that protects them against depolymerization. The stabilization of the newly severed plus ends and the higher rescue frequency synergize to amplify microtubule number and mass. Thus, severing enzymes regulate microtubule architecture and dynamics by promoting GTP-tubulin incorporation within the microtubule shaft. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000442828700036 |
WOS关键词 | GUANOSINE 5-TRIPHOSPHATE HYDROLYSIS ; PROMOTES MICROTUBULE ; DYNAMIC INSTABILITY ; MEIOTIC SPINDLE ; SELF-REPAIR ; IN-VIVO ; KATANIN ; SPASTIN ; PROTEIN ; PLUS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199420 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.NINDS, Cell Biol & Biophys Unit, Porter Neurosci Res Ctr, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA; 2.Brandeis Univ, Howard Hughes Med Inst, Waltham, MA 02454 USA; 3.Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02903 USA; 4.NHLBI, Biochem & Biophys Ctr, Bldg 10, Bethesda, MD 20892 USA; 5.Howard Hughes Med Inst, Janelia Farm Res Campus, Ashburn, VA 20147 USA |
推荐引用方式 GB/T 7714 | Vemu, Annapurna,Szczesna, Ewa,Zehr, Elena A.,et al. Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation[J]. SCIENCE,2018,361(6404). |
APA | Vemu, Annapurna.,Szczesna, Ewa.,Zehr, Elena A..,Spector, Jeffrey O..,Grigorieff, Nikolaus.,...&Roll-Mecak, Antonina.(2018).Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation.SCIENCE,361(6404). |
MLA | Vemu, Annapurna,et al."Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation".SCIENCE 361.6404(2018). |
条目包含的文件 | 条目无相关文件。 |
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