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DOI | 10.1126/science.aat5641 |
Three-dimensional genome structures of single diploid human cells | |
Tan, Longzhi1,2; Xing, Dong1; Chang, Chi-Han1; Li, Heng3; Xie, Sunney1,4,5 | |
2018-08-31 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6405页码:924-928 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | Three-dimensional genome structures play a key role in gene regulation and cell functions. Characterization of genome structures necessitates single-cell measurements. This has been achieved for haploid cells but has remained a challenge for diploid cells. We developed a single-cell chromatin conformation capture method, termed Dip-C, that combines a transposon-based whole-genome amplification method to detect many chromatin contacts, called META (multiplex end-tagging amplification), and an algorithm to impute the two chromosome haplotypes linked by each contact. We reconstructed the genome structures of single diploid human cells from a lymphoblastoid cell line and from primary blood cells with high spatial resolution, locating specific single-nucleotide and copy number variations in the nucleus. The two alleles of imprinted loci and the two X chromosomes were structurally different. Cells of different types displayed statistically distinct genome structures. Such structural cell typing is crucial for understanding cell functions. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000443547000040 |
WOS关键词 | INACTIVE X-CHROMOSOME ; HI-C ; CHROMATIN ACCESSIBILITY ; NUCLEAR ARCHITECTURE ; REVEALS PRINCIPLES ; ORGANIZATION ; REORGANIZATION ; LYMPHOCYTES ; RESOLUTION ; DOMAINS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199477 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 2.Harvard Med Sch, Syst Biol PhD Program, Boston, MA 02115 USA; 3.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA; 4.Peking Univ, Innovat Ctr Genom, Beijing 100871, Peoples R China; 5.Peking Univ, Biodynam & Opt Imaging Ctr, Beijing 100871, Peoples R China |
推荐引用方式 GB/T 7714 | Tan, Longzhi,Xing, Dong,Chang, Chi-Han,et al. Three-dimensional genome structures of single diploid human cells[J]. SCIENCE,2018,361(6405):924-928. |
APA | Tan, Longzhi,Xing, Dong,Chang, Chi-Han,Li, Heng,&Xie, Sunney.(2018).Three-dimensional genome structures of single diploid human cells.SCIENCE,361(6405),924-928. |
MLA | Tan, Longzhi,et al."Three-dimensional genome structures of single diploid human cells".SCIENCE 361.6405(2018):924-928. |
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