Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat5062 |
Programmable protein circuits in living cells | |
Gao, Xiaojing J.; Chong, Lucy S.; Kim, Matthew S.; Elowitz, Michael B. | |
2018-09-21 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6408页码:1252-1258 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Synthetic protein-level circuits could enable engineering of powerful new cellular behaviors. Rational protein circuit design would be facilitated by a composable protein-protein regulation system in which individual protein components can regulate one another to create a variety of different circuit architectures. In this study, we show that engineered viral proteases can function as composable protein components, which can together implement a broad variety of circuit-level functions in mammalian cells. In this system, termed CHOMP (circuits of hacked orthogonal modular proteases), input proteases dock with and cleave target proteases to inhibit their function. These components can be connected to generate regulatory cascades, binary logic gates, and dynamic analog signal-processing functions. To demonstrate the utility of this system, we rationally designed a circuit that induces cell death in response to upstream activators of the Ras oncogene. Because CHOMP circuits can perform complex functions yet be encoded as single transcripts and delivered without genomic integration, they offer a scalable platform to facilitate protein circuit engineering for biotechnological applications. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000445152500042 |
WOS关键词 | HEPATITIS-C VIRUS ; SYNTHETIC BIOLOGY ; IN-VIVO ; PATHWAYS ; ACTIVATION ; DESIGN ; IDENTIFICATION ; CONSTRUCTION ; FLUORESCENT ; SUFFICIENT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199626 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | CALTECH, Howard Hughes Med Inst, Div Biol & Biol Engn, Broad Ctr, 1200 East Calif Blvd, Pasadena, CA 91125 USA |
推荐引用方式 GB/T 7714 | Gao, Xiaojing J.,Chong, Lucy S.,Kim, Matthew S.,et al. Programmable protein circuits in living cells[J]. SCIENCE,2018,361(6408):1252-1258. |
APA | Gao, Xiaojing J.,Chong, Lucy S.,Kim, Matthew S.,&Elowitz, Michael B..(2018).Programmable protein circuits in living cells.SCIENCE,361(6408),1252-1258. |
MLA | Gao, Xiaojing J.,et al."Programmable protein circuits in living cells".SCIENCE 361.6408(2018):1252-1258. |
条目包含的文件 | 条目无相关文件。 |
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