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DOI | 10.1126/science.aaq0681 |
Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration | |
Gerber, Tobias1; Murawala, Prayag2,3,6; Knapp, Dunja3; Masselink, Wouter2,6; Schuez, Maritta3; Hermann, Sarah3; Gac-Santel, Malgorzata1; Nowoshilow, Sergej2,3,6; Kageyama, Jorge1; Khattak, Shahryar3; Currie, Joshua D.3,7; Camp, J. Gray1; Tanaka, Elly M.2,3,6; Treutlein, Barbara1,4,5 | |
2018-10-26 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6413页码:421-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Austria; Canada |
英文摘要 | Amputation of the axolotl forelimb results in the formation of a blastema, a transient tissue where progenitor cells accumulate prior to limb regeneration. However, the molecular understanding of blastema formation had previously been hampered by the inability to identify and isolate blastema precursor cells in the adult tissue. We have used a combination of Cre-loxP reporter lineage tracking and single-cell messenger RNA sequencing (scRNA-seq) to molecularly track mature connective tissue (CT) cell heterogeneity and its transition to a limb blastema state. We have uncovered a multiphasic molecular program where CT cell types found in the uninjured adult limb revert to a relatively homogenous progenitor state that recapitulates an embryonic limb bud-like phenotype including multipotency within the CT lineage. Together, our data illuminate molecular and cellular reprogramming during complex organ regeneration in a vertebrate. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450441900040 |
WOS关键词 | LINEAGE ANALYSIS ; GENE-EXPRESSION ; RNA-SEQ ; TISSUE ; ENHANCER ; BUD ; SMART-SEQ2 ; ELEMENTS ; REVEALS ; ORIGIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199935 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, Deutsch Pl 6, D-04103 Leipzig, Germany; 2.Vienna BioCtr VBC, Res Inst Mol Pathol IMP, Campus Vienna Bioctr 1, A-1030 Vienna, Austria; 3.Tech Univ Dresden, DFG, Ctr Regenerat Therapies CRTD, Fetscherstr 105, D-01307 Dresden, Germany; 4.Max Planck Inst Mol Cell Biol & Genet, 108 Pfotenhauerstr, D-01307 Dresden, Germany; 5.Tech Univ Munich, Dept Biosci, D-85354 Freising Weihenstephan, Germany; 6.IMP, Campus Vienna Bioctr 1, A-1030 Vienna, Austria; 7.Univ Toronto, Dept Cell & Syst Biol, 25 Harbord St, Toronto, ON M5S 3G5, Canada |
推荐引用方式 GB/T 7714 | Gerber, Tobias,Murawala, Prayag,Knapp, Dunja,et al. Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration[J]. SCIENCE,2018,362(6413):421-+. |
APA | Gerber, Tobias.,Murawala, Prayag.,Knapp, Dunja.,Masselink, Wouter.,Schuez, Maritta.,...&Treutlein, Barbara.(2018).Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration.SCIENCE,362(6413),421-+. |
MLA | Gerber, Tobias,et al."Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration".SCIENCE 362.6413(2018):421-+. |
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