Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat6678 |
Structural insight into precursor tRNA processing by yeast ribonuclease P | |
Lan, Pengfei1; Tan, Ming2,3; Zhang, Yuebin4; Niu, Shuangshuang2,3,5; Chen, Juan1; Shi, Shaohua1; Qiu, Shuwan6,7; Wang, Xuejuan6,7; Peng, Xiangda4; Cai, Gang6,7; Cheng, Hong2; Wu, Jian1; Li, Guohui4; Lei, Ming1,8,9,10 | |
2018-11-09 | |
发表期刊 | SCIENCE
![]() |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6415页码:657-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe). The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S(N)2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450474500033 |
WOS关键词 | PRE-TRANSFER-RNA ; DENSITY-FUNCTIONAL THERMOCHEMISTRY ; METAL-BINDING SITE ; MOLECULAR-DYNAMICS ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; 2-METAL-ION MECHANISM ; RIBOZYME CATALYSIS ; FORCE-FIELD ; C5 PROTEIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200033 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China; 2.Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China; 4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 6.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China; 7.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China; 8.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China; 9.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China; 10.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China |
推荐引用方式 GB/T 7714 | Lan, Pengfei,Tan, Ming,Zhang, Yuebin,et al. Structural insight into precursor tRNA processing by yeast ribonuclease P[J]. SCIENCE,2018,362(6415):657-+. |
APA | Lan, Pengfei.,Tan, Ming.,Zhang, Yuebin.,Niu, Shuangshuang.,Chen, Juan.,...&Lei, Ming.(2018).Structural insight into precursor tRNA processing by yeast ribonuclease P.SCIENCE,362(6415),657-+. |
MLA | Lan, Pengfei,et al."Structural insight into precursor tRNA processing by yeast ribonuclease P".SCIENCE 362.6415(2018):657-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论