GSTDTAP  > 地球科学
DOI10.1126/science.aat6678
Structural insight into precursor tRNA processing by yeast ribonuclease P
Lan, Pengfei1; Tan, Ming2,3; Zhang, Yuebin4; Niu, Shuangshuang2,3,5; Chen, Juan1; Shi, Shaohua1; Qiu, Shuwan6,7; Wang, Xuejuan6,7; Peng, Xiangda4; Cai, Gang6,7; Cheng, Hong2; Wu, Jian1; Li, Guohui4; Lei, Ming1,8,9,10
2018-11-09
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2018
卷号362期号:6415页码:657-+
文章类型Article
语种英语
国家Peoples R China
英文摘要

Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe). The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S(N)2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000450474500033
WOS关键词PRE-TRANSFER-RNA ; DENSITY-FUNCTIONAL THERMOCHEMISTRY ; METAL-BINDING SITE ; MOLECULAR-DYNAMICS ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; 2-METAL-ION MECHANISM ; RIBOZYME CATALYSIS ; FORCE-FIELD ; C5 PROTEIN
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/200033
专题地球科学
资源环境科学
气候变化
作者单位1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China;
2.Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China;
3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China;
4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China;
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China;
6.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China;
7.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China;
8.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China;
9.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China;
10.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China
推荐引用方式
GB/T 7714
Lan, Pengfei,Tan, Ming,Zhang, Yuebin,et al. Structural insight into precursor tRNA processing by yeast ribonuclease P[J]. SCIENCE,2018,362(6415):657-+.
APA Lan, Pengfei.,Tan, Ming.,Zhang, Yuebin.,Niu, Shuangshuang.,Chen, Juan.,...&Lei, Ming.(2018).Structural insight into precursor tRNA processing by yeast ribonuclease P.SCIENCE,362(6415),657-+.
MLA Lan, Pengfei,et al."Structural insight into precursor tRNA processing by yeast ribonuclease P".SCIENCE 362.6415(2018):657-+.
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