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DOI | 10.1126/science.aar7981 |
Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation | |
El Meouche, Imane1,2,3; Dunlop, Mary J.1,2,3 | |
2018-11-09 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6415页码:686-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Antibiotic resistance is often the result of mutations that block drug activity; however, bacteria also evade antibiotics by transiently expressing genes such as multidrug efflux pumps. A crucial question is whether transient resistance can promote permanent genetic changes. Previous studies have established that antibiotic treatment can select tolerant cells that then mutate to achieve permanent resistance. Whether these mutations result from antibiotic stress or preexist within the population is unclear. To address this question, we focused on the multidrug pump AcrAB-TolC. Using time-lapse microscopy, we found that cells with higher acrAB expression have lower expression of the DNA mismatch repair gene mutS, lower growth rates, and higher mutation frequencies. Thus, transient antibiotic resistance from elevated acrAB expression can promote spontaneous mutations within single cells. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450474500041 |
WOS关键词 | ESCHERICHIA-COLI K-12 ; COMPLETE GENOME SEQUENCE ; FLUOROQUINOLONE RESISTANCE ; CHROMOSOMAL GENES ; BACTERIAL-CELLS ; MISMATCH REPAIR ; WEAK MUTATORS ; EVOLUTION ; TOLERANCE ; ACRB |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200038 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA; 2.Boston Univ, Biol Design Ctr, Boston, MA 02215 USA; 3.Univ Vermont, Burlington, VT 05405 USA |
推荐引用方式 GB/T 7714 | El Meouche, Imane,Dunlop, Mary J.. Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation[J]. SCIENCE,2018,362(6415):686-+. |
APA | El Meouche, Imane,&Dunlop, Mary J..(2018).Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation.SCIENCE,362(6415),686-+. |
MLA | El Meouche, Imane,et al."Heterogeneity in efflux pump expression predisposes antibiotic-resistant cells to mutation".SCIENCE 362.6415(2018):686-+. |
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