Cap-specific terminal N-6-methylation of RNA by an RNA polymerase II-associated methyltransferase

doi:10.1126/science.aav0080

GSTDTAP > 地球科学

DOI	10.1126/science.aav0080
	Cap-specific terminal N-6-methylation of RNA by an RNA polymerase II-associated methyltransferase
	Akichika, Shinichiro 1; Hirano, Seiichi 2; Shichino, Yuichi 3; Suzuki, Takeo 1; Nishimasu, Hiroshi 2; Ishitani, Ryuichiro 2; Sugita, Ai 4; Hirose, Yutaka 4; Iwasaki, Shintaro 3,5; Nureki, Osamu 2; Suzuki, Tsutomu 1
	2019-01-11
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2019
卷号	363 期号:6423 页码:141-141
文章类型	Article
语种	英语
国家	Japan
英文摘要	N-6-methyladenosine (m(6)A), a major modification of messenger RNAs (mRNAs), plays critical roles in RNA metabolism and function. In addition to the internal m(6)A, N-6, 2'-O-dimethyladenosine (m(6)Am) is present at the transcription start nucleotide of capped mRNAs in vertebrates. However, its biogenesis and functional role remain elusive. Using a reverse genetics approach, we identified PCIF1, a factor that interacts with the serine-5-phosphorylated carboxyl-terminal domain of RNA polymerase II, as a cap-specific adenosine methyltransferase (CAPAM) responsible for N-6-methylation of m(6)Am. The crystal structure of CAPAM in complex with substrates revealed the molecular basis of cap-specific m(6)A formation. A transcriptome-wide analysis revealed that N-6-methylation of m(6)Am promotes the translation of capped mRNAs. Thus, a cap-specific m(6)A writer promotes translation of mRNAs starting from m(6)Am.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000455320600038
WOS关键词	MESSENGER-RNA ; GENE-EXPRESSION ; TRANSLATION INITIATION ; METHYLATION ; M(6)A ; BINDING ; PROTEIN ; N-6-METHYLADENOSINE ; TRANSCRIPTION ; MECHANISMS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/200503
专题	地球科学资源环境科学气候变化
作者单位	1.Univ Tokyo, Dept Chem & Biotechnol, Grad Sch Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan; 2.Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan; 3.RIKEN, Cluster Pioneering Res, RNA Syst Biochem Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan; 4.Univ Toyama, Grad Sch Med & Pharmaceut Sci, Lab Gene Regulat, 2630 Sugitani, Toyama 9300194, Japan; 5.Univ Tokyo, Grad Sch Frontier Sci, Dept Computat Biol & Med Sci, Tokyo 2778562, Japan
推荐引用方式 GB/T 7714	Akichika, Shinichiro,Hirano, Seiichi,Shichino, Yuichi,et al. Cap-specific terminal N-6-methylation of RNA by an RNA polymerase II-associated methyltransferase[J]. SCIENCE,2019,363(6423):141-141.
APA	Akichika, Shinichiro.,Hirano, Seiichi.,Shichino, Yuichi.,Suzuki, Takeo.,Nishimasu, Hiroshi.,...&Suzuki, Tsutomu.(2019).Cap-specific terminal N-6-methylation of RNA by an RNA polymerase II-associated methyltransferase.SCIENCE,363(6423),141-141.
MLA	Akichika, Shinichiro,et al."Cap-specific terminal N-6-methylation of RNA by an RNA polymerase II-associated methyltransferase".SCIENCE 363.6423(2019):141-141.