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DOI | 10.1126/science.aav8912 |
Structural insight into nucleosome transcription by RNA polymerase II with elongation factors | |
Ehara, Haruhiko1; Kujirai, Tomoya1,2; Fujino, Yuka2,3; Shirouzu, Mikako1; Kurumizaka, Hitoshi1,2,3; Sekine, Shun-ichi1 | |
2019-02-15 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6428页码:744-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Japan |
英文摘要 | RNA polymerase II (RNAPII) transcribes chromosomal DNA that contains multiple nucleosomes. The nucleosome forms transcriptional barriers, and nucleosomal transcription requires several additional factors in vivo. We demonstrate that the transcription elongation factors Elf1 and Spt4/5 cooperatively lower the barriers and increase the RNAPII processivity in the nucleosome. The cryo-electron microscopy structures of the nucleosome-transcribing RNAPII elongation complexes (ECs) reveal that Elf1 and Spt4/5 reshape the EC downstream edge and intervene between RNAPII and the nucleosome. They facilitate RNAPII progression through superhelical location SHL(-1) by adjusting the nucleosome in favor of the forward progression. They suppress pausing at SHL(-5) by preventing the stable RNAPII-nucleosome interaction. Thus, the EC overcomes the nucleosomal barriers while providing a platform for various chromatin functions. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000458874100034 |
WOS关键词 | CRYSTAL-STRUCTURE ; COMPLEX ; MECHANISM ; SPT5 ; VISUALIZATION ; INITIATION ; PARTICLE ; BARRIER ; DSIF |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200760 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.RIKEN, Ctr Biosyst Dynam Res, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan; 2.Univ Tokyo, Inst Quantitat Biosci, Lab Chromatin Struct & Funct, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1130032, Japan; 3.Waseda Univ, Grad Sch Adv Sci & Engn, Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan |
推荐引用方式 GB/T 7714 | Ehara, Haruhiko,Kujirai, Tomoya,Fujino, Yuka,et al. Structural insight into nucleosome transcription by RNA polymerase II with elongation factors[J]. SCIENCE,2019,363(6428):744-+. |
APA | Ehara, Haruhiko,Kujirai, Tomoya,Fujino, Yuka,Shirouzu, Mikako,Kurumizaka, Hitoshi,&Sekine, Shun-ichi.(2019).Structural insight into nucleosome transcription by RNA polymerase II with elongation factors.SCIENCE,363(6428),744-+. |
MLA | Ehara, Haruhiko,et al."Structural insight into nucleosome transcription by RNA polymerase II with elongation factors".SCIENCE 363.6428(2019):744-+. |
条目包含的文件 | 条目无相关文件。 |
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