Structural insight into nucleosome transcription by RNA polymerase II with elongation factors

doi:10.1126/science.aav8912

GSTDTAP > 地球科学

DOI	10.1126/science.aav8912
	Structural insight into nucleosome transcription by RNA polymerase II with elongation factors
	Ehara, Haruhiko 1; Kujirai, Tomoya 1,2; Fujino, Yuka 2,3; Shirouzu, Mikako 1; Kurumizaka, Hitoshi 1,2,3; Sekine, Shun-ichi 1
	2019-02-15
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2019
卷号	363 期号:6428 页码:744-+
文章类型	Article
语种	英语
国家	Japan
英文摘要	RNA polymerase II (RNAPII) transcribes chromosomal DNA that contains multiple nucleosomes. The nucleosome forms transcriptional barriers, and nucleosomal transcription requires several additional factors in vivo. We demonstrate that the transcription elongation factors Elf1 and Spt4/5 cooperatively lower the barriers and increase the RNAPII processivity in the nucleosome. The cryo-electron microscopy structures of the nucleosome-transcribing RNAPII elongation complexes (ECs) reveal that Elf1 and Spt4/5 reshape the EC downstream edge and intervene between RNAPII and the nucleosome. They facilitate RNAPII progression through superhelical location SHL(-1) by adjusting the nucleosome in favor of the forward progression. They suppress pausing at SHL(-5) by preventing the stable RNAPII-nucleosome interaction. Thus, the EC overcomes the nucleosomal barriers while providing a platform for various chromatin functions.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000458874100034
WOS关键词	CRYSTAL-STRUCTURE ; COMPLEX ; MECHANISM ; SPT5 ; VISUALIZATION ; INITIATION ; PARTICLE ; BARRIER ; DSIF
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/200760
专题	地球科学资源环境科学气候变化
作者单位	1.RIKEN, Ctr Biosyst Dynam Res, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan; 2.Univ Tokyo, Inst Quantitat Biosci, Lab Chromatin Struct & Funct, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1130032, Japan; 3.Waseda Univ, Grad Sch Adv Sci & Engn, Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan
推荐引用方式 GB/T 7714	Ehara, Haruhiko,Kujirai, Tomoya,Fujino, Yuka,et al. Structural insight into nucleosome transcription by RNA polymerase II with elongation factors[J]. SCIENCE,2019,363(6428):744-+.
APA	Ehara, Haruhiko,Kujirai, Tomoya,Fujino, Yuka,Shirouzu, Mikako,Kurumizaka, Hitoshi,&Sekine, Shun-ichi.(2019).Structural insight into nucleosome transcription by RNA polymerase II with elongation factors.SCIENCE,363(6428),744-+.
MLA	Ehara, Haruhiko,et al."Structural insight into nucleosome transcription by RNA polymerase II with elongation factors".SCIENCE 363.6428(2019):744-+.