Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aaw1219 |
Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution | |
Rodriques, Samuel G.1,2,3; Stickels, Robert R.3,4,5; Goeva, Aleksandrina3; Martin, Carly A.3; Murray, Evan3; Vanderburg, Charles R.3; Welch, Joshua3; Chen, Linlin M.3; Chen, Fei3; Macosko, Evan Z.3,6 | |
2019-03-29 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 363期号:6434页码:1463-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Spatial positions of cells in tissues strongly influence function, yet a high-throughput, genome-wide readout of gene expression with cellular resolution is lacking. We developed Slide-seq, a method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, allowing the locations of the RNA to be inferred by sequencing. Using Slide-seq, we localized cell types identified by single-cell RNA sequencing datasets within the cerebellum and hippocampus, characterized spatial gene expression patterns in the Purkinje layer of mouse cerebellum, and defined the temporal evolution of cell type-specific responses in a mouse model of traumatic brain injury. These studies highlight how Slide-seq provides a scalable method for obtaining spatially resolved gene expression data at resolutions comparable to the sizes of individual cells. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000462738000039 |
WOS关键词 | CEREBELLAR CORTEX ; GENE-EXPRESSION ; CELLS ; RAT ; ORGANIZATION ; RNA ; VESTIBULOCEREBELLUM ; COMPARTMENTS ; ACTIVATION ; DIVERSITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201074 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.MIT, Dept Phys, Cambridge, MA 02139 USA; 2.MIT, Media Lab, Cambridge, MA 02139 USA; 3.Broad Inst Harvard & MIT, Cambridge, MA 02142 USA; 4.Harvard Univ, Grad Sch Arts & Sci, Cambridge, MA 02138 USA; 5.Harvard Med Sch, Div Med Sci, Boston, MA 02115 USA; 6.Massachusetts Gen Hosp, Dept Psychiat, Boston, MA 02114 USA |
推荐引用方式 GB/T 7714 | Rodriques, Samuel G.,Stickels, Robert R.,Goeva, Aleksandrina,et al. Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution[J]. SCIENCE,2019,363(6434):1463-+. |
APA | Rodriques, Samuel G..,Stickels, Robert R..,Goeva, Aleksandrina.,Martin, Carly A..,Murray, Evan.,...&Macosko, Evan Z..(2019).Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution.SCIENCE,363(6434),1463-+. |
MLA | Rodriques, Samuel G.,et al."Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution".SCIENCE 363.6434(2019):1463-+. |
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