GSTDTAP  > 地球科学
DOI10.1126/science.aav7541
Packing of apolar side chains enables accurate design of highly stable membrane proteins
Mravic, Marco1; Thomaston, Jessica L.1; Tucker, Maxwell1; Solomon, Paige E.1; Liu, Lijun2,3; DeGrado, William F.1
2019-03-29
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2019
卷号363期号:6434页码:1418-+
文章类型Article
语种英语
国家USA; Peoples R China
英文摘要

The features that stabilize the structures of membrane proteins remain poorly understood. Polar interactions contribute modestly, and the hydrophobic effect contributes little to the energetics of apolar side-chain packing in membranes. Disruption of steric packing can destabilize the native folds of membrane proteins, but is packing alone sufficient to drive folding in lipids? If so, then membrane proteins stabilized by this feature should be readily designed and structurally characterized-yet this has not been achieved. Through simulation of the natural protein phospholamban and redesign of variants, we define a steric packing code underlying its assembly. Synthetic membrane proteins designed using this code and stabilized entirely by apolar side chains conform to the intended fold. Although highly stable, the steric complementarity required for their folding is surprisingly stringent. Structural informatics shows that the designed packing motif recurs across the proteome, emphasizing a prominent role for precise apolar packing in membrane protein folding, stabilization, and evolution.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000462738000029
WOS关键词DE-NOVO DESIGN ; TRANSMEMBRANE HELIX ; COMPUTATIONAL DESIGN ; PHOSPHOLAMBAN PENTAMER ; CYSTEINE RESIDUES ; SELF-ASSOCIATION ; POLAR RESIDUES ; ALPHA-HELICES ; COILED-COILS ; STABILITY
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/201076
专题地球科学
资源环境科学
气候变化
作者单位1.Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA;
2.Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Shenzhen 518055, Peoples R China;
3.DLX Sci, Lawrence, KS 66049 USA
推荐引用方式
GB/T 7714
Mravic, Marco,Thomaston, Jessica L.,Tucker, Maxwell,et al. Packing of apolar side chains enables accurate design of highly stable membrane proteins[J]. SCIENCE,2019,363(6434):1418-+.
APA Mravic, Marco,Thomaston, Jessica L.,Tucker, Maxwell,Solomon, Paige E.,Liu, Lijun,&DeGrado, William F..(2019).Packing of apolar side chains enables accurate design of highly stable membrane proteins.SCIENCE,363(6434),1418-+.
MLA Mravic, Marco,et al."Packing of apolar side chains enables accurate design of highly stable membrane proteins".SCIENCE 363.6434(2019):1418-+.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Mravic, Marco]的文章
[Thomaston, Jessica L.]的文章
[Tucker, Maxwell]的文章
百度学术
百度学术中相似的文章
[Mravic, Marco]的文章
[Thomaston, Jessica L.]的文章
[Tucker, Maxwell]的文章
必应学术
必应学术中相似的文章
[Mravic, Marco]的文章
[Thomaston, Jessica L.]的文章
[Tucker, Maxwell]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。