GSTDTAP  > 地球科学
DOI10.1126/science.aav5870
Reconstitution and structure of a plant NLR resistosome conferring immunity
Wang, Jizong1; Hu, Meijuan2; Wang, Jia1; Qi, Jinfeng2; Han, Zhifu1; Wang, Guoxun2; Qi, Yijun1; Wang, Hong-Wei1; Zhou, Jian-Min2; Chai, Jijie1,3,4
2019-04-05
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2019
卷号364期号:6435页码:44-+
文章类型Article
语种英语
国家Peoples R China; Germany
英文摘要

Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an a-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic a helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000463585700029
WOS关键词CRYO-EM STRUCTURE ; RUST RESISTANCE PROTEIN ; DOMAIN-LIKE PROTEIN ; DISEASE RESISTANCE ; CELL-DEATH ; CRYSTAL-STRUCTURE ; COILED-COIL ; CASPASE-9 ACTIVATION ; PATHOGEN PERCEPTION ; NUCLEOTIDE-BINDING
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/201130
专题地球科学
资源环境科学
气候变化
作者单位1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Ctr Plant Biol,Sch Life Sci, Beijing 100084, Peoples R China;
2.Chinese Acad Sci, Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China;
3.Max Planck Inst Plant Breeding Res, Cologne, Germany;
4.Univ Cologne, Inst Biochem, Zuelpicher Str 47, D-50674 Cologne, Germany
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GB/T 7714
Wang, Jizong,Hu, Meijuan,Wang, Jia,et al. Reconstitution and structure of a plant NLR resistosome conferring immunity[J]. SCIENCE,2019,364(6435):44-+.
APA Wang, Jizong.,Hu, Meijuan.,Wang, Jia.,Qi, Jinfeng.,Han, Zhifu.,...&Chai, Jijie.(2019).Reconstitution and structure of a plant NLR resistosome conferring immunity.SCIENCE,364(6435),44-+.
MLA Wang, Jizong,et al."Reconstitution and structure of a plant NLR resistosome conferring immunity".SCIENCE 364.6435(2019):44-+.
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