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DOI | 10.1126/science.aav5870 |
Reconstitution and structure of a plant NLR resistosome conferring immunity | |
Wang, Jizong1; Hu, Meijuan2; Wang, Jia1; Qi, Jinfeng2; Han, Zhifu1; Wang, Guoxun2; Qi, Yijun1; Wang, Hong-Wei1; Zhou, Jian-Min2; Chai, Jijie1,3,4 | |
2019-04-05 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6435页码:44-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; Germany |
英文摘要 | Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an a-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic a helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000463585700029 |
WOS关键词 | CRYO-EM STRUCTURE ; RUST RESISTANCE PROTEIN ; DOMAIN-LIKE PROTEIN ; DISEASE RESISTANCE ; CELL-DEATH ; CRYSTAL-STRUCTURE ; COILED-COIL ; CASPASE-9 ACTIVATION ; PATHOGEN PERCEPTION ; NUCLEOTIDE-BINDING |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201130 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Ctr Plant Biol,Sch Life Sci, Beijing 100084, Peoples R China; 2.Chinese Acad Sci, Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 3.Max Planck Inst Plant Breeding Res, Cologne, Germany; 4.Univ Cologne, Inst Biochem, Zuelpicher Str 47, D-50674 Cologne, Germany |
推荐引用方式 GB/T 7714 | Wang, Jizong,Hu, Meijuan,Wang, Jia,et al. Reconstitution and structure of a plant NLR resistosome conferring immunity[J]. SCIENCE,2019,364(6435):44-+. |
APA | Wang, Jizong.,Hu, Meijuan.,Wang, Jia.,Qi, Jinfeng.,Han, Zhifu.,...&Chai, Jijie.(2019).Reconstitution and structure of a plant NLR resistosome conferring immunity.SCIENCE,364(6435),44-+. |
MLA | Wang, Jizong,et al."Reconstitution and structure of a plant NLR resistosome conferring immunity".SCIENCE 364.6435(2019):44-+. |
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