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DOI | 10.1126/science.aax3289 |
Mechanism of 5 ' splice site transfer for human spliceosome activation | |
Charenton, Clement; Wilkinson, Max E.; Nagai, Kiyoshi | |
2019-04-26 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6438页码:362-+ |
文章类型 | Article |
语种 | 英语 |
国家 | England |
英文摘要 | The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs) bound to the precursor messenger RNA 5'splice site (5'SS) and branch point sequence, associates with the U4/U6. U5 tri-snRNP to form the fully assembled precatalytic pre-B spliceosome. Here, we report cryo-electron microscopy structures of the human pre-B complex captured before U1 snRNP dissociation at 3.3-angstrom core resolution and the human tri-snRNP at 2.9-angstrom resolution. U1 snRNP inserts the 5'SS-U1 snRNA helix between the two RecA domains of the Prp28 DEAD-box helicase. Adenosine 5'-triphosphate-dependent closure of the Prp28 RecA domains releases the 5'SS to pair with the nearby U6 ACAGAGA-box sequence presented as a mobile loop. The structures suggest that formation of the 5'SS-ACAGAGA helix triggers remodeling of an intricate protein-RNA network to induce Brr2 helicase relocation to its loading sequence in U4 snRNA, enabling Brr2 to unwind the U4/U6 snRNA duplex to allow U6 snRNA to form the catalytic center of the spliceosome. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000465643400041 |
WOS关键词 | U4/U6.U5 TRI-SNRNP ; CRYO-EM STRUCTURE ; CRYSTAL-STRUCTURE ; STRUCTURAL BASIS ; REQUIRES ATP ; RNA ; PROTEIN ; ARCHITECTURE ; RESOLUTION ; NUCLEAR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201281 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | MRC Lab Mol Biol, Cambridge CB2 0QH, England |
推荐引用方式 GB/T 7714 | Charenton, Clement,Wilkinson, Max E.,Nagai, Kiyoshi. Mechanism of 5 ' splice site transfer for human spliceosome activation[J]. SCIENCE,2019,364(6438):362-+. |
APA | Charenton, Clement,Wilkinson, Max E.,&Nagai, Kiyoshi.(2019).Mechanism of 5 ' splice site transfer for human spliceosome activation.SCIENCE,364(6438),362-+. |
MLA | Charenton, Clement,et al."Mechanism of 5 ' splice site transfer for human spliceosome activation".SCIENCE 364.6438(2019):362-+. |
条目包含的文件 | 条目无相关文件。 |
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