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DOI | 10.1126/science.aav2572 |
Particulate methane monooxygenase contains only mononuclear copper centers | |
Ross, Matthew O.1,2; MacMillan, Fraser3; Wang, Jingzhou4,5; Nisthal, Alex4,5,7; Lawton, Thomas J.1,2,8; Olafson, Barry D.6; Mayo, Stephen L.4,5; Rosenzweig, Amy C.1,2; Hoffman, Brian M.1,2 | |
2019-05-10 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6440页码:566-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | Bacteria that oxidize methane to methanol are central to mitigating emissions of methane, a potent greenhouse gas. The nature of the copper active site in the primary metabolic enzyme of these bacteria, particulate methane monooxygenase (pMMO), has been controversial owing to seemingly contradictory biochemical, spectroscopic, and crystallographic results. We present biochemical and electron paramagnetic resonance spectroscopic characterization most consistent with two monocopper sites within pMMO: one in the soluble PmoB subunit at the previously assigned active site (Cu-B) and one similar to 2 nanometers away in the membrane-bound PmoC subunit (Cu-C). On the basis of these results, we propose that a monocopper site is able to catalyze methane oxidation in pMMO. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000467631800038 |
WOS关键词 | METHYLOCOCCUS-CAPSULATUS BATH ; COMPREHENSIVE SOFTWARE PACKAGE ; FOLDING QUALITY-CONTROL ; CRYSTAL-STRUCTURE ; NATURAL-GAS ; HYDROGEN-PEROXIDE ; COMPLEXES ; EPR ; ENDOR ; COORDINATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201397 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Northwestern Univ, Dept Mol Biosci, 2205 Tech Dr, Evanston, IL 60208 USA; 2.Northwestern Univ, Dept Chem, 2145 Sheridan Rd, Evanston, IL 60208 USA; 3.Univ East Anglia, Sch Chem, Henry Wellcome Unit Biol Electron Paramagnet Reso, Norwich NR4 7TJ, Norfolk, England; 4.CALTECH, Div Biol, MC 114-96,1200 East Calif Blvd, Pasadena, CA 91125 USA; 5.CALTECH, Div Chem & Chem Engn, MC 114-96,1200 East Calif Blvd, Pasadena, CA 91125 USA; 6.Protabit, 1010 E Union St,Suite 110, Pasadena, CA 91106 USA; 7.Xencor, 111 W Lemon Ave, Monrovia, CA 91016 USA; 8.Johns Hopkins Appl Phys Lab, 11100 Johns Hopkins Rd, Laurel, MD 20723 USA |
推荐引用方式 GB/T 7714 | Ross, Matthew O.,MacMillan, Fraser,Wang, Jingzhou,et al. Particulate methane monooxygenase contains only mononuclear copper centers[J]. SCIENCE,2019,364(6440):566-+. |
APA | Ross, Matthew O..,MacMillan, Fraser.,Wang, Jingzhou.,Nisthal, Alex.,Lawton, Thomas J..,...&Hoffman, Brian M..(2019).Particulate methane monooxygenase contains only mononuclear copper centers.SCIENCE,364(6440),566-+. |
MLA | Ross, Matthew O.,et al."Particulate methane monooxygenase contains only mononuclear copper centers".SCIENCE 364.6440(2019):566-+. |
条目包含的文件 | 条目无相关文件。 |
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