Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aau3644 |
Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments | |
Hafner, Anne-Sophie1; Donlin-Asp, Paul G.1; Leitch, Beulah2,3; Herzog, Etienne4,5; Schuman, Erin M.1 | |
2019-05-17 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6441页码:650-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; New Zealand; France |
英文摘要 | There is ample evidence for localization of messenger RNAs (mRNAs) and protein synthesis in neuronal dendrites; however, demonstrations of these processes in presynaptic terminals are limited. We used expansion microscopy to resolve pre- and postsynaptic compartments in rodent neurons. Most presynaptic terminals in the hippocampus and forebrain contained mRNA and ribosomes. We sorted fluorescently labeled mouse brain synaptosomes and then sequenced hundreds of mRNA species present within excitatory boutons. After brief metabolic labeling, >30% of all presynaptic terminals exhibited a signal, providing evidence for ongoing protein synthesis. We tested different classic plasticity paradigms and observed distinct patterns of rapid pre- and/or postsynaptic translation. Thus, presynaptic terminals are translationally competent, and local protein synthesis is differentially recruited to drive compartment-specific phenotypes that underlie different forms of plasticity. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000468551000024 |
WOS关键词 | MESSENGER-RNAS ; SYNAPTIC-TRANSMISSION ; GENE-EXPRESSION ; VESICULAR GABA ; TRANSLATION ; TRANSPORT ; IDENTIFICATION ; VISUALIZATION ; REPERTOIRE ; PLASTICITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201449 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Brain Res, Frankfurt, Germany; 2.Univ Otago, Sch Biomed Sci, Dept Anat, Dunedin, New Zealand; 3.Univ Otago, Brain Hlth Res Ctr, Dunedin, New Zealand; 4.Univ Bordeaux, Interdisciplinary Inst Neurosci, UMR5297, F-33000 Bordeaux, France; 5.CNRS, UMR 5297, Interdisciplinary Inst Neurosci, F-33000 Bordeaux, France |
推荐引用方式 GB/T 7714 | Hafner, Anne-Sophie,Donlin-Asp, Paul G.,Leitch, Beulah,et al. Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments[J]. SCIENCE,2019,364(6441):650-+. |
APA | Hafner, Anne-Sophie,Donlin-Asp, Paul G.,Leitch, Beulah,Herzog, Etienne,&Schuman, Erin M..(2019).Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments.SCIENCE,364(6441),650-+. |
MLA | Hafner, Anne-Sophie,et al."Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments".SCIENCE 364.6441(2019):650-+. |
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