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DOI | 10.1126/science.aav6390 |
Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer | |
Nolivos, Sophie1,3; Cayron, Julien1; Dedieu, Annick1; Page, Adeline2; Delolme, Frederic2; Lesterlin, Christian1 | |
2019-05-24 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 364期号:6442页码:778-+ |
文章类型 | Article |
语种 | 英语 |
国家 | France |
英文摘要 | Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor-conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000469296000044 |
WOS关键词 | FLUORESCENT-PROTEIN REPORTER ; ESCHERICHIA-COLI ; ANTIBIOTIC-RESISTANCE ; GENE RECOMBINATION ; CHROMOSOME ; MECHANISMS ; SURVIVAL ; MUTATION ; SYSTEM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/201483 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Lyon 1, CNRS, UMR5086, INSERM,MMSB, F-69007 Lyon, France; 2.UCBL, INSERM, CNRS, Prot Sci Facil,SFR BioSci,UMS3444,US8,ENS Lyon, F-69007 Lyon, France; 3.Univ Pau & Pays Adour, Inst Sci Analyt & Physicochim Environm & Mat IPRE, CNRS, UMR5254,E2S UPPA, F-64000 Pau, France |
推荐引用方式 GB/T 7714 | Nolivos, Sophie,Cayron, Julien,Dedieu, Annick,et al. Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer[J]. SCIENCE,2019,364(6442):778-+. |
APA | Nolivos, Sophie,Cayron, Julien,Dedieu, Annick,Page, Adeline,Delolme, Frederic,&Lesterlin, Christian.(2019).Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer.SCIENCE,364(6442),778-+. |
MLA | Nolivos, Sophie,et al."Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer".SCIENCE 364.6442(2019):778-+. |
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