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DOI | 10.1126/science.aaw9144 |
Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase | |
Zhou, Long; Sazanov, Leonid A. | |
2019-08-23 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6455页码:773-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Austria |
英文摘要 | V (vacuolar)/A (archaeal)-type adenosine triphosphatases (ATPases), found in archaea and eubacteria, couple ATP hydrolysis or synthesis to proton translocation across the plasma membrane using the rotary-catalysis mechanism. They belong to the V-type ATPase family. which differs from the mitochondrial/chloroplast F-type ATP synthases in overall architecture. We solved cryo-electron microscopy structures of the intact Therm us thermophilus V/A-ATPase, reconstituted into lipid nanodiscs, in three rotational states and two substates. These structures indicate substantial flexibility between V-1 and V-o in a working enzyme. which results from mechanical competition between central shaft rotation and resistance from the peripheral stalks. We also describe details of adenosine diphosphate inhibition release, V-1-V-o torque transmission, and proton translocation, which are relevant for the entire V-type ATPase family. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000482464000043 |
WOS关键词 | BEAM-INDUCED MOTION ; CRYSTAL-STRUCTURE ; V-ATPASE ; CRYO-EM ; BOVINE F-1-ATPASE ; ATOMIC MODEL ; SUBUNIT ; COMPLEX ; STALK ; MECHANISM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202136 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | IST Austria, A-3400 Klosterneuburg, Austria |
推荐引用方式 GB/T 7714 | Zhou, Long,Sazanov, Leonid A.. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase[J]. SCIENCE,2019,365(6455):773-+. |
APA | Zhou, Long,&Sazanov, Leonid A..(2019).Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase.SCIENCE,365(6455),773-+. |
MLA | Zhou, Long,et al."Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase".SCIENCE 365.6455(2019):773-+. |
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