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DOI | 10.1126/science.aay0737 |
Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly | |
Wang, Kaihang1,2; de la Torre, Daniel1; Robertson, Wesley E.1; Chin, Jason W.1 | |
2019-08-30 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6456页码:922-+ |
文章类型 | Article |
语种 | 英语 |
国家 | England; USA |
英文摘要 | The design and creation of synthetic genomes provide a powerful approach to understanding and engineering biology. However, it is often limited by the paucity of methods for precise genome manipulation. Here, we demonstrate the programmed fission of the Escherichia coli genome into diverse pairs of synthetic chromosomes and the programmed fusion of synthetic chromosomes to generate genomes with user-defined inversions and translocations. We further combine genome fission, chromosome transplant, and chromosome fusion to assemble genomic regions from different strains into a single genome. Thus, we program the scarless assembly of new genomes with nucleotide precision, a key step in the convergent synthesis of genomes from diverse progenitors. This work provides a set of precise, rapid, large-scale (megabase) genome-engineering operations for creating diverse synthetic genomes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000483975800047 |
WOS关键词 | ESCHERICHIA-COLI |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202187 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.MRC, Mol Biol Lab, Francis Crick Ave, Cambridge, England; 2.CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA |
推荐引用方式 GB/T 7714 | Wang, Kaihang,de la Torre, Daniel,Robertson, Wesley E.,et al. Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly[J]. SCIENCE,2019,365(6456):922-+. |
APA | Wang, Kaihang,de la Torre, Daniel,Robertson, Wesley E.,&Chin, Jason W..(2019).Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly.SCIENCE,365(6456),922-+. |
MLA | Wang, Kaihang,et al."Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly".SCIENCE 365.6456(2019):922-+. |
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