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DOI | 10.1126/science.aaw0542 |
Rock-paper-scissors: Engineered population dynamics increase genetic stability | |
Liao, Michael J.1,2; Din, M. Omar2; Tsinring, Lev2,3; Hasty, Jeff1,2,3,4 | |
2019-09-06 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6457页码:1045-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Advances in synthetic biology have led to an arsenal of proof-of-principle bacterial circuits that can be leveraged for applications ranging from therapeutics to bioproduction. A unifying challenge for most applications is the presence of selective pressures that lead to high mutation rates for engineered bacteria. A common strategy is to develop cloning technologies aimed at increasing the fixation time for deleterious mutations in single cells. We adopt a complementary approach that is guided by ecological interactions, whereby cyclical population control is engineered to stabilize the functionality of intracellular gene circuits. Three strains of Escherichia coli were designed such that each strain could kill or be killed by one of the other two strains. The resulting "rock-paper-scissors" dynamic demonstrates rapid cycling of strains in microfluidic devices and leads to an increase in the stability of gene circuit functionality in cell culture. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000484732700051 |
WOS关键词 | SYNTHETIC BIOLOGY ; CONSTRUCTION ; ORGANISMS ; PROMOTERS ; BACTERIA ; GAME |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202249 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA; 2.Univ Calif San Diego, BioCircuits Inst, La Jolla, CA 92093 USA; 3.Univ Calif San Diego, San Diego Ctr Syst Biol, San Diego, CA 92103 USA; 4.Univ Calif San Diego, Div Biol Sci, Mol Biol Sect, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Liao, Michael J.,Din, M. Omar,Tsinring, Lev,et al. Rock-paper-scissors: Engineered population dynamics increase genetic stability[J]. SCIENCE,2019,365(6457):1045-+. |
APA | Liao, Michael J.,Din, M. Omar,Tsinring, Lev,&Hasty, Jeff.(2019).Rock-paper-scissors: Engineered population dynamics increase genetic stability.SCIENCE,365(6457),1045-+. |
MLA | Liao, Michael J.,et al."Rock-paper-scissors: Engineered population dynamics increase genetic stability".SCIENCE 365.6457(2019):1045-+. |
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