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DOI | 10.1126/science.aax7852 |
CRISPR-mediated live imaging of genome editing and transcription | |
Wang, Haifeng1; Nakamura, Muneaki1; Abbott, Timothy R.1; Zhao, Dehua1; Luo, Kaiwen1,2; Yu, Cordelia1,3; Nguyen, Cindy M.1; Lo, Albert1; Daley, Timothy P.1,4; La Russa, Marie1; Liu, Yanxia1; Qi, Lei S.1,5,6 | |
2019-09-20 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6459页码:1301-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | We report a robust, versatile approach called CRISPR live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad range of cell types, including primary cells. An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromosomal disorders such as Patau syndrome in prenatal amniotic fluid cells and track multiple loci in human T lymphocytes. In addition, LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9-mediated editing and consequent chromosome translocations. Finally, by combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. The LiveFISH approach enables real-time live imaging of DNA and RNA during genome editing, transcription, and rearrangements in single cells. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000487547400046 |
WOS关键词 | GENES ; LOCI ; DYNAMICS ; PLATFORM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202348 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; 2.Zhejiang Univ, ZJU UOE Inst, Sch Med, Haining, Peoples R China; 3.Castilleja Sch, Palo Alto, CA 94301 USA; 4.Stanford Univ, Dept Stat, Stanford, CA 94305 USA; 5.Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA; 6.Stanford Univ, ChEM H Inst, Stanford, CA 94305 USA |
推荐引用方式 GB/T 7714 | Wang, Haifeng,Nakamura, Muneaki,Abbott, Timothy R.,et al. CRISPR-mediated live imaging of genome editing and transcription[J]. SCIENCE,2019,365(6459):1301-+. |
APA | Wang, Haifeng.,Nakamura, Muneaki.,Abbott, Timothy R..,Zhao, Dehua.,Luo, Kaiwen.,...&Qi, Lei S..(2019).CRISPR-mediated live imaging of genome editing and transcription.SCIENCE,365(6459),1301-+. |
MLA | Wang, Haifeng,et al."CRISPR-mediated live imaging of genome editing and transcription".SCIENCE 365.6459(2019):1301-+. |
条目包含的文件 | 条目无相关文件。 |
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