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DOI | 10.1126/science.aax4804 |
Ultrasound imaging of gene expression in mammalian cells | |
Farhadi, Arash1; Ho, Gabrielle H.2,3; Sawyer, Daniel P.1; Bourdeau, Raymond W.2,4; Shapiro, Mikhail G.2 | |
2019-09-27 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 365期号:6460页码:1469-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in living animals. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000488838600052 |
WOS关键词 | REPORTER GENES ; PROTEIN ; PLATFORM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202403 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA; 2.CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA; 3.Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA; 4.Codiak Biosci, Cambridge, MA USA |
推荐引用方式 GB/T 7714 | Farhadi, Arash,Ho, Gabrielle H.,Sawyer, Daniel P.,et al. Ultrasound imaging of gene expression in mammalian cells[J]. SCIENCE,2019,365(6460):1469-+. |
APA | Farhadi, Arash,Ho, Gabrielle H.,Sawyer, Daniel P.,Bourdeau, Raymond W.,&Shapiro, Mikhail G..(2019).Ultrasound imaging of gene expression in mammalian cells.SCIENCE,365(6460),1469-+. |
MLA | Farhadi, Arash,et al."Ultrasound imaging of gene expression in mammalian cells".SCIENCE 365.6460(2019):1469-+. |
条目包含的文件 | 条目无相关文件。 |
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