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DOI | 10.1038/nature25474 |
A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis | |
Gorbea, Carlos1; Mosbruger, Tim2; Cazalla, Demian1 | |
2017-10-12 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 550期号:7675页码:275-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Viruses express several classes of non-coding RNAs1; the functions and mechanisms by which most of these act are unknown. Herpesvirus saimiri, a gamma-herpesvirus that establishes latency in the T cells of New World primates and has the ability to cause aggressive leukaemias and lymphomas in non-natural hosts(2), expresses seven small nuclear uracil-rich non-coding RNAs (called HSURs) in latently infected cells(3-5). These HSURs associate with Sm proteins, and share biogenesis and structural features with cellular Sm-class small nuclear RNAs4,6. One of these HSURs (HSUR2) base-pairs with two host cellular microRNAs (miR-142-3p and miR-16(7)) but does not affect their abundance or activity, which suggests that its interactions with them perform alternative functions. Here we show that HSUR2 also base-pairs with mRNAs in infected cells. We combined in vivo psoralen-mediated RNA-RNA crosslinking and high-throughput sequencing to identify the mRNAs targeted by HSUR2, which include mRNAs that encode retinoblastoma and factors involved in p53 signalling and apoptosis. We show that HSUR2 represses the expression of target mRNAs and that base pairing between HSUR2 and miR-142-3p and miR-16 is essential for this repression, suggesting that HSUR2 recruits these two cellular microRNAs to its target mRNAs. Furthermore, we show that HSUR2 uses this mechanism to inhibit apoptosis. Our results uncover a role for this viral Sm-class RNA as a microRNA adaptor in the regulation of gene expression that follows precursor mRNA processing. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000412829500052 |
WOS关键词 | SMALL NUCLEAR RNAS ; HERPESVIRUS-SAIMIRI ; NONCODING RNAS ; DELETION MUTANTS ; RECOMBINATION ; MECHANISMS ; PATHWAY ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/202842 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA; 2.Univ Utah, Sch Med, Huntsman Canc Inst, Salt Lake City, UT 84112 USA |
推荐引用方式 GB/T 7714 | Gorbea, Carlos,Mosbruger, Tim,Cazalla, Demian. A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis[J]. NATURE,2017,550(7675):275-+. |
APA | Gorbea, Carlos,Mosbruger, Tim,&Cazalla, Demian.(2017).A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.NATURE,550(7675),275-+. |
MLA | Gorbea, Carlos,et al."A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis".NATURE 550.7675(2017):275-+. |
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