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DOI | 10.1038/s41586-019-1748-4 |
Marine Proteobacteria metabolize glycolate via the 尾-hydroxyaspartate cycle | |
Lennart Schada von Borzyskowski; Francesca Severi; Karen Kr眉ger; Lucas Hermann; Alexandre Gilardet; Felix Sippel; Bianca Pommerenke; Peter Claus; Ni帽a Socorro Cortina; Timo Glatter; Stefan Zauner; Jan Zarzycki; Bernhard M. Fuchs; Erhard Bremer; Uwe G. Maier; Rudolf I. Amann; Tobias J. Erb | |
2019-11-13 | |
发表期刊 | Nature |
出版年 | 2019 |
卷号 | 575页码:500-504 |
英文摘要 | One of the most abundant sources of organic carbon in the ocean is glycolate, the secretion of which by marine phytoplankton results in an estimated annual flux of one petagram of glycolate in marine environments1. Although it is generally accepted that glycolate is oxidized to glyoxylate by marine bacteria2,3,4, the further fate of this C2 metabolite is not well understood. Here we show that ubiquitous marine Proteobacteria are able to assimilate glyoxylate via the 尾-hydroxyaspartate cycle (BHAC) that was originally proposed 56聽years ago5. We elucidate the biochemistry of the BHAC and describe the structure of its key enzymes, including a previously unknown primary imine reductase. Overall, the BHAC enables the direct production of oxaloacetate from glyoxylate through only four enzymatic steps, representing鈥攖o our knowledge鈥攖he most efficient glyoxylate assimilation route described to date. Analysis of marine metagenomes shows that the BHAC is globally distributed and on average 20-fold more abundant than the glycerate pathway, the only other known pathway for net glyoxylate assimilation. In a field study of a phytoplankton bloom, we show that glycolate is present in high nanomolar concentrations and taken up by prokaryotes at rates that allow a full turnover of the glycolate pool within one week. During the bloom, genes that encode BHAC key enzymes are present in up to 1.5% of the bacterial community and actively transcribed, supporting the role of the BHAC in glycolate assimilation and suggesting a previously undescribed trophic interaction between autotrophic phytoplankton and heterotrophic bacterioplankton. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203152 |
专题 | 地球科学 资源环境科学 气候变化 |
推荐引用方式 GB/T 7714 | Lennart Schada von Borzyskowski,Francesca Severi,Karen Kr眉ger,等. Marine Proteobacteria metabolize glycolate via the 尾-hydroxyaspartate cycle[J]. Nature,2019,575:500-504. |
APA | Lennart Schada von Borzyskowski.,Francesca Severi.,Karen Kr眉ger.,Lucas Hermann.,Alexandre Gilardet.,...&Tobias J. Erb.(2019).Marine Proteobacteria metabolize glycolate via the 尾-hydroxyaspartate cycle.Nature,575,500-504. |
MLA | Lennart Schada von Borzyskowski,et al."Marine Proteobacteria metabolize glycolate via the 尾-hydroxyaspartate cycle".Nature 575(2019):500-504. |
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