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DOI | 10.1038/ncomms14377 |
RNA structure drives interaction with proteins | |
de Groot, Natalia Sanchez1; Armaos, Alexandros1; Grana-Montes, Ricardo1,8; Alriquet, Marion2,3; Calloni, Giulia2,3; Vabulas, R. Martin2,3; Tartaglia, Gian Gaetano1,4,5,6,7 | |
2019-07-19 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Spain; Germany; Italy; Switzerland |
英文摘要 | The combination of high-throughput sequencing and in vivo crosslinking approaches leads to the progressive uncovering of the complex interdependence between cellular transcriptome and proteome. Yet, the molecular determinants governing interactions in protein-RNA networks are not well understood. Here we investigated the relationship between the structure of an RNA and its ability to interact with proteins. Analysing in silico, in vitro and in vivo experiments, we find that the amount of double-stranded regions in an RNA correlates with the number of protein contacts. This relationship -which we call structure-driven protein interactivity- allows classification of RNA types, plays a role in gene regulation and could have implications for the formation of phase-separated ribonucleoprotein assemblies. We validate our hypothesis by showing that a highly structured RNA can rearrange the composition of a protein aggregate. We report that the tendency of proteins to phase-separate is reduced by interactions with specific RNAs. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000476471300054 |
WOS关键词 | CELL-FREE FORMATION ; SECONDARY STRUCTURE ; PHASE-SEPARATION ; BINDING PROTEINS ; FUNCTIONAL ANNOTATION ; WEB SERVER ; DISCOVERY ; IDENTIFICATION ; PREDICTIONS ; MECHANISMS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203215 |
专题 | 资源环境科学 |
作者单位 | 1.Barcelona Inst Sci & Technol, CRG, Dr Aiguader 88, Barcelona 08003, Spain; 2.Goethe Univ Frankfurt, Buchmann Inst Mol Life Sci, D-60438 Frankfurt, Germany; 3.Goethe Univ Frankfurt, Inst Biophys Chem, D-60438 Frankfurt, Germany; 4.ICREA, 23 Passeig Lluis Co, Barcelona 08010, Spain; 5.Univ Pompeu Fabra, Barcelona 08003, Spain; 6.Sapienza Univ Rome, Dept Biol Charles Darwin, Ple A Moro 5, I-00185 Rome, Italy; 7.Ist Italiano Tecnol, Dept Neurosci & Brain Technol, Via Morego 30, I-16163 Genoa, Italy; 8.Univ Zurich, Dept Biochem, Winterthurerstr 190, CH-8057 Zurich, Switzerland |
推荐引用方式 GB/T 7714 | de Groot, Natalia Sanchez,Armaos, Alexandros,Grana-Montes, Ricardo,et al. RNA structure drives interaction with proteins[J]. NATURE COMMUNICATIONS,2019,10. |
APA | de Groot, Natalia Sanchez.,Armaos, Alexandros.,Grana-Montes, Ricardo.,Alriquet, Marion.,Calloni, Giulia.,...&Tartaglia, Gian Gaetano.(2019).RNA structure drives interaction with proteins.NATURE COMMUNICATIONS,10. |
MLA | de Groot, Natalia Sanchez,et al."RNA structure drives interaction with proteins".NATURE COMMUNICATIONS 10(2019). |
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